Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio.
Genetic basis of thiaminase I activity in a vertebrate, zebrafish Danio rerio.
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DOI：
10.1038/s41598-023-27612-5
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发表时间：
2023-01-13
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
--
作者：
硫胺素（维生素B1）代谢是人类和动物健康和生态功能的重要驱动因素。一些生物，包括蕨类植物、软体动物和鱼类，含有硫胺素降解酶，即硫胺素酶，食用这些生物会导致食用者体内硫胺素缺乏。食用含有硫胺酶的鱼类已导致世界各地养殖动物和野生鲑鱼种群的死亡率升高和招募失败。在北美五大湖，本地湖鳟鱼（Salvelinus namaycush）食用非本地猎物灰鲱（Alosa pseudoharengus）导致鳟鱼体内硫胺素缺乏，导致鱼苗死亡率升高，并阻碍了自然种群的补充。在细菌和单细胞真核生物中已经发现了几种硫胺酶的遗传特征，多细胞生物中硫胺酶的来源被假设为肠道菌群。在一个意想不到的发现中，我们在斑马鱼（Danio rerio）中发现了与细菌tenA硫胺酶II同源的硫胺酶I基因。在重组体系中证实了斑马鱼硫胺酶I （GenBank NP_001314821.1）的生化活性。在鲤鱼（Cyprinus carpio）、斑马贻贝（Dreissena polymorpha）和灰鲭鱼（alewife）等多种动物中发现了与斑马鱼tena样硫胺酶I同源的基因。因此，影响湖鳟鱼种群的灰鲱体内硫胺酶I的来源很可能是从头合成的。
Thiamine (vitamin B1) metabolism is an important driver of human and animal health and ecological functioning. Some organisms, including species of ferns, mollusks, and fish, contain thiamine-degrading enzymes known as thiaminases, and consumption of these organisms can lead to thiamine deficiency in the consumer. Consumption of fish containing thiaminase has led to elevated mortality and recruitment failure in farmed animals and wild salmonine populations around the world. In the North American Great Lakes, consumption of the non-native prey fish alewife (Alosa pseudoharengus) by native lake trout (Salvelinus namaycush) led to thiamine deficiency in the trout, contributed to elevated fry mortality, and impeded natural population recruitment. Several thiaminases have been genetically characterized in bacteria and unicellular eukaryotes, and the source of thiaminase in multicellular organisms has been hypothesized to be gut microflora. In an unexpected discovery, we identified thiaminase I genes in zebrafish (Danio rerio) with homology to bacterial tenA thiaminase II. The biochemical activity of zebrafish thiaminase I (GenBank NP_001314821.1) was confirmed in a recombinant system. Genes homologous to the zebrafish tenA-like thiaminase I were identified in many animals, including common carp (Cyprinus carpio), zebra mussel (Dreissena polymorpha) and alewife. Thus, the source of thiaminase I in alewife impacting lake trout populations is likely to be de novo synthesis.
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影响因子：
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