Comparative Microbiomics Analysis of Antimicrobial Antibody Response between Patients with Lung Cancer and Control Subjects with Benign Pulmonary Nodules.
Comparative Microbiomics Analysis of Antimicrobial Antibody Response between Patients with Lung Cancer and Control Subjects with Benign Pulmonary Nodules.
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DOI：
10.1158/1055-9965.epi-22-0384
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发表时间：
2023-04-03
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
计算机断层扫描（CT）筛查可以早期发现肺癌，但假阳性率高。CT扫描发现的不确定肺结节（IPN），需要能够区分其良恶性的分子生物标志物。我们利用蛋白微阵列技术对127例肺腺癌（ADC）、123例吸烟者对照（SMC）和170例良性结节对照（BNC）患者血清中27种细菌和29种病毒的901种微生物抗原进行了抗体分析，以鉴定ADC和BNC特异性抗微生物抗体。分析第4个四分位数比值比，我们发现3个BNC亚组中抗体的患病率高于ADC或SMC。我们发现，与SMC相比，抗幽门螺杆菌抗体在ADC中的患病率更高。我们进行了亚组分析，发现轻度吸烟者（≤20包-年）比重度吸烟者（≤20包-年）、结节大小为bb1 1cm的BNC比结节<= 1cm的BNC患病率更高、I期ADC比II期和III期ADC患病率更高。我们进行了多变量分析，并构建了能够区分ADC与SMC和ADC与BNC的抗体面板，其受试者工作特征曲线下面积（AUC）分别为0.88和0.80。抗微生物抗体有可能降低CT筛查的假阳性率，并为肺癌的发展提供有趣的见解。微生物感染在肺癌的发展和良性肺结节的形成中起着重要的作用。
Computer Tomography (CT) screening can detect lung cancer early but suffers a high false positive rate. There is a need for molecular biomarkers that can distinguish malignant and benign indeterminate pulmonary nodules (IPN) detected by CT scan. We profiled antibodies against 901 individual microbial antigens from 27 bacteria and 29 viruses in sera from 127 lung adenocarcinoma (ADC), 123 smoker controls (SMC), 170 benign nodule controls (BNC) individuals using protein microarrays to identify ADC and BNC specific anti-microbial antibodies. Analyzing 4th quartile odds ratios, we found more antibodies with higher prevalence in the 3 BNC subgroups than in ADC or SMC. We demonstrated that significantly more anti-Helicobacter pylori antibodies showed higher prevalence in ADC relative to SMC. We performed subgroup analysis and found that more antibodies with higher prevalence in light smokers (≤ 20 pack-years) compared with heavy smokers (> 20 pack-years), in BNC with nodule size > 1cm than in those with <= 1cm nodules, and in stage I ADC than in stage II and III ADC. We performed multivariate analysis and constructed antibody panels that can distinguish ADC vs. SMC and ADC vs. BNC with area under the receiver operating characteristics curve (AUC) of 0.88 and 0.80, respectively. Anti-microbial antibodies have the potential to reduce the false positive rate of CT screening and provide interesting insight in lung cancer development. Microbial infection plays an important role in lung cancer development and the formation of benign pulmonary nodules.
影响因子：
3.7
作者：
Koshiol J;Flores R;Lam TK;Taylor PR;Weinstein SJ;Virtamo J;Albanes D;Perez-Perez G;Caporaso NE;Blaser MJ
通讯作者：
Blaser MJ
影响因子：
8.8
作者：
Anderson, K. S.;Wong, J.;D'Souza, G.;Riemer, A. B.;Lorch, J.;Haddad, R.;Pai, S. I.;Longtine, J.;McClean, M.;LaBaer, J.;Kelsey, K. T.;Posner, M.
通讯作者：
Posner, M.
影响因子：
0.6
作者：
Samareh Fekri M;Hashemi Bajgani SM;Rasti A;Yazdani R;Mollaie HR
通讯作者：
Mollaie HR