Elucidation of the molecular responses of a cucumber segment substitution line carrying Pm5.1 and its recurrent parent triggered by powdery mildew by comparative transcriptome profiling.
Elucidation of the molecular responses of a cucumber segment substitution line carrying Pm5.1 and its recurrent parent triggered by powdery mildew by comparative transcriptome profiling.
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通过比较转录组分析阐明白粉病引发的携带 Pm5.1 的黄瓜片段替换系及其轮回亲本的分子反应
DOI：
10.1186/s12864-016-3438-z
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发表时间：
2017-01-05
期刊：
影响因子：
4.4
通讯作者：
Chen X
中科院分区：
文献类型：
--
作者：
Xu Q;Xu X;Shi Y;Qi X;Chen X
背景：白粉病是瓜类最严重的真菌病害之一，但黄瓜抗白粉病的分子机制尚不清楚。本研究利用黄瓜抗晚疫病自交系D8的分子标记辅助回交技术，从抗晚疫病供体金5 -508的第5染色体上分离出一个代表Pm 5. 1基因的片段，并构建了一个抗晚疫病片段代换系SSL 508 - 28。这导致鉴定出一个约6.8 Mb的取代片段，预计包含856个基因。RNA-seq用于研究PM处理的（接种后48小时收获的植物）和未处理的（对照）SSL 508 -28和D8系中的基因表达差异。在SSL 508 -28和D8中分别鉴定了1，248和1，325个差异表达基因（DEG）。其中，88个DEG位于~6.8 Mb段间隔内。基于表达数据和注释，我们鉴定了8个可能参与Pm5.1提供的PM抗性的潜在候选基因，包括两个串联排列的编码受体蛋白激酶的基因、两个转录因子的基因、两个编码Pm5.1蛋白的基因、一个编码P型ATP酶的基因和一个编码70 kDa热休克蛋白的基因。转录组数据还揭示了Pm5.1介导的PM抗性的复杂调控网络，可能涉及多个信号调节子和转导子、细胞壁修饰和水杨酸信号通路。结论：这些发现揭示了Pm5.1介导的黄瓜PM防御机制，为Pm5.1基因的精细定位和黄瓜PM抗性育种提供了有价值的信息。
Background:Powdery mildew (PM) is one of the most severe fungal diseases of cucurbits, but the molecular mechanisms underlying PM resistance in cucumber remain elusive. In this study, we developed a PM resistant segment substitution line SSL508-28 that carried a segment on chromosome five representing the Pm5.1 locus from PM resistant donor Jin5-508 using marker-assisted backcrossing of an elite PM susceptible cucumber inbred line D8.Results:Whole-genome resequencing of SSL508-28, Jin5-508 and D8 was performed to identify the exact boundaries of the breakpoints for this introgression because of the low density of available single sequence repeat markers. This led to the identification of a ~6.8 Mb substituted segment predicted to contain 856 genes. RNA-seq was used to study gene expression differences in PM treated (plants harvested 48 h after inoculation) and untreated (control) SSL508-28 and D8 lines. Exactly 1,248 and 1,325 differentially expressed genes (DEGs) were identified in SSL508-28 and D8, respectively. Of those, 88 DEGs were located in the ~6.8 Mb segment interval. Based on expression data and annotation, we identified 8 potential candidate genes that may participate in PM resistance afforded by Pm5.1, including two tandemly arrayed genes encoding receptor protein kinases, two transcription factors, two genes encoding remorin proteins, one gene encoding a P-type ATPase and one gene encoding a 70 kDa heat shock protein. The transcriptome data also revealed a complex regulatory network for Pm5.1-mediated PM resistance that may involve multiple signal regulators and transducers, cell wall modifications and the salicylic acid signaling pathway.Conclusion:These findings shed light on the cucumber PM defense mechanisms mediated by Pm5.1 and provided valuable information for the fine mapping of Pm5.1 and breeding of cucumber with enhanced resistance to PM.
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影响因子：
56.9
作者：
DELANEY, TP;UKNES, S;RYALS, J
通讯作者：
RYALS, J
影响因子：
64.8
作者：
Gan X;Stegle O;Behr J;Steffen JG;Drewe P;Hildebrand KL;Lyngsoe R;Schultheiss SJ;Osborne EJ;Sreedharan VT;Kahles A;Bohnert R;Jean G;Derwent P;Kersey P;Belfield EJ;Harberd NP;Kemen E;Toomajian C;Kover PX;Clark RM;Rätsch G;Mott R
通讯作者：
Mott R
DOI：
10.1073/pnas.0606330103
发表时间：
2006-11-14
影响因子：
11.1
作者：
Fauteux, Francois;Chain, Florian;Belanger, Richard R.
通讯作者：
Belanger, Richard R.
影响因子：
1.2
作者：
Cingolani, Pablo;Platts, Adrian;Ruden, Douglas M.
通讯作者：
Ruden, Douglas M.
影响因子：
5.4
作者：
He, Xiaoming;Li, Yuhong;Weng, Yiqun
通讯作者：
Weng, Yiqun