Identification of Serum CMTM2 as a Potential Biomarker for HBV-Related Disorders.
Identification of Serum CMTM2 as a Potential Biomarker for HBV-Related Disorders.
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鉴定血清 CMTM2 作为 HBV 相关疾病的潜在生物标志物
DOI：
10.1155/2020/2032056
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发表时间：
2020
期刊：
影响因子：
--
通讯作者：
Chen WX
中科院分区：
文献类型：
--
作者：
Chen S;Hu Q;Chen H;Zhang FF;Duan L;Wang B;Li DD;Zhang J;Chen WX
大量的进展支持CMTM 2在生理和病理过程中充当重要的执行者。然而，CMTM 2与HBV相关疾病之间的关系尚不清楚。在此，我们首次探讨血清CMTM 2是否参与HBV相关疾病。我们发现，使用ELISA测定，与健康对照相比，患者中的CMTM 2值显著较低（p <0.001）。慢性乙型肝炎患者血清CMTM 2水平与HBV DNA水平呈负相关，与ALT、AST水平无相关性。HBLC和HCC患者血清CMTM 2浓度与血清ALT、AST及HBV DNA载量无相关性。此外，ROC曲线分析表明，CMTM 2水平与HBV相关疾病的诊断价值显著相关。最后，在HBV感染的细胞模型中观察到CMTM 2的下调。CMTM 2降解可归因于HBx激活的Lys 48（K48）连接的多聚泛素化，其可通过蛋白酶体抑制剂MG 132处理而消除。HBV感染通过激活泛素-蛋白酶体系统抑制CMTM 2表达。血清CMTM 2水平可作为HBV相关疾病发病机制的有效指标。
Substantial advance supports that CMTM2 serve as an important performer in physiological and pathological processes. However, very little is clear about the relationship between CMTM2 and HBV-related disorders. Here, for the first time, we explore that whether or not serum CMTM2 is involved in HBV-related diseases. We found that CMTM2 values were significantly lower in patients compared to healthy control (p <0.001), using ELISA assay. Furthermore, serum CMTM2 levels were negatively correlated with HBV DNA levels in CHB patients but not correlated with the serum levels of ALT and AST. Serum CMTM2 concentrations were not correlated with the serum levels of ALT, AST and HBV DNA load in HBLC and HCC patients. In addition, analysis of the ROC curve indicated that CMTM2 levels were significantly associated with the diagnostic value of HBV-related disorders. Finally, downregulation of CMTM2 was observed in HBV-infected cell model. CMTM2 degradation could be attributed to HBx-activated Lys48 (K48)-linked polyubiquitination, which was abolished by treatment with the proteasome inhibitor MG132. HBV infection suppresses CMTM2 expression by activating ubiquitin-proteasome system. Serum CMTM2 levels can be adopted as an effective indicator of the pathogenesis of HBV-related disorders.
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