Newly expressed progesterone receptor cannot activate stable, replicated mouse mammary tumor virus templates but acquires transactivation potential upon continuous expression.
Newly expressed progesterone receptor cannot activate stable, replicated mouse mammary tumor virus templates but acquires transactivation potential upon continuous expression.
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新表达的黄体酮受体不能激活稳定的、复制的小鼠乳腺肿瘤病毒模板，但在连续表达后获得反式激活潜力。
DOI：
10.1073/pnas.90.23.11202
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发表时间：
1993
影响因子：
11.1
通讯作者：
Hager,GL
中科院分区：
文献类型：
--
作者：
Smith,CL;Archer,TK;Hamlin-Green,G;Hager,GL
During development and differentiation, the expression of transcription factors is regulated in a temporal fashion. Newly expressed transcription factors must interact productively with target genes organized in chromatin. Although the mechanisms governing factor binding to chromatin templates are not well understood, it is now clear that template access can be dramatically influenced by nucleoprotein structure. We have examined the ability of a well characterized transactivator, the progesterone receptor (PR), to activate the mouse mammary tumor virus (MMTV) promoter organized either in stable, replicating templates that have a highly ordered nucleosome structure or as transiently transfected DNA, which adopts a less-defined structure. If the PR is transiently expressed in cells harboring both replicated and transient MMTV receptor constructs, it cannot significantly activate the stable replicated MMTV template. In contrast, when PR cDNA is stably inserted into the same cells and constitutively expressed, it gains the ability to activate both chromosomal and transiently introduced templates. These results demonstrate that newly expressed PR is not competent to activate the MMTV template in its native nucleoprotein conformation but acquires this ability upon prolonged expression in replicating cells.
糖皮质激素和孕激素受体结合到两个激素调节启动子的相同位点
影响因子：
64.8
作者：
D. V. D. Ahe;S. Janich;C. Scheidereit;R. Renkawitz;G. Schütz;M. Beato
通讯作者：
M. Beato
小鼠乳腺肿瘤病毒长末端重复序列的两个区域调节其启动子在乳腺细胞系中的活性
影响因子：
5.3
作者：
P. Lefebvre;D. Berard;M. Cordingley;and;G. Hager
通讯作者：
G. Hager