Transposon sequencing analysis of Bradyrhizobium diazoefficiens 110spc4.
Transposon sequencing analysis of Bradyrhizobium diazoefficiens 110spc4.
复制标题
重氮慢生根瘤菌 110spc4 的转座子测序分析
DOI：
10.1038/s41598-021-92534-z
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发表时间：
2021-06-24
影响因子：
4.6
通讯作者：
Harwood CS
中科院分区：
文献类型：
--
作者：
Baraquet C;Dai W;Mendiola J;Pechter K;Harwood CS
重氮效率慢生根瘤菌USDA110是大豆最有效的固氮共生体之一。本研究对源自USDA110的菌株Bd110spc4进行了大规模转座子插入测序（n-seq）分析，目的是增加对该植物共生体在不同条件下生存至关重要的基因的可用资源。我们制备了Bd110spc4的两个转座子（Tn）插入文库，当文库组合时，有155,042个独特的Tn插入，平均每58.7 bp插入一个参考USDA110基因组。应用生物信息学过滤步骤去除太小而不可能有Tn插入的基因，产生了一个被归类为推定必需基因的基因列表。将这组基因与与α -变形菌密切相关的红假单胞菌（Rhodopseudomonas palustris）生长所必需的基因进行比较，发现了一个由5个基因组成的小集合，这些基因可能是与慢根瘤菌科密切相关的成员所必需的。这一群体包括生活方式多样的细菌，从植物共生体到动物相关物种，再到自由生活物种。
Bradyrhizobium diazoefficiens USDA110 is one of the most effective nitrogen-fixing symbionts of soybeans. Here we carried out a large-scale transposon insertion sequencing (Tn-seq) analysis of strain Bd110spc4, which is derived from USDA110, with the goal of increasing available resources for identifying genes crucial for the survival of this plant symbiont under diverse conditions. We prepared two transposon (Tn) insertion libraries of Bd110spc4 with 155,042 unique Tn insertions when the libraries were combined, which is an average of one insertion every 58.7 bp of the reference USDA110 genome. Application of bioinformatic filtering steps to remove genes too small to be expected to have Tn insertions, resulted in a list of genes that were classified as putatively essential. Comparison of this gene set with genes putatively essential for the growth of the closely related alpha-proteobacterium, Rhodopseudomonas palustris, revealed a small set of five genes that may be collectively essential for closely related members of the family Bradyrhizobiaceae. This group includes bacteria with diverse lifestyles ranging from plant symbionts to animal-associated species to free-living species.
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影响因子：
9.9
作者：
通讯作者：
--
DOI：
10.1038/nrmicro.2017.173
发表时间：
2018-05
期刊：
Nature reviews. Microbiology
影响因子：
--
作者：
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通讯作者：
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影响因子：
4.1
作者：
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通讯作者：
Tabata, S
影响因子：
7
作者：
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通讯作者：
Turner, A. Keith
DOI：
10.1073/pnas.1519220112
发表时间：
2015-12-01
影响因子：
11.1
作者：
Rubin, Benjamin E.;Wetmore, Kelly M.;Golden, Susan S.
通讯作者：
Golden, Susan S.