Ultrasound-triggered release of materials entrapped in microbubble-liposome constructs: a tool for targeted drug delivery.
Ultrasound-triggered release of materials entrapped in microbubble-liposome constructs: a tool for targeted drug delivery.
复制标题
DOI：
10.1016/j.jconrel.2010.07.115
复制
发表时间：
2010-11-20
期刊：
影响因子：
--
通讯作者：
Chin CT
中科院分区：
文献类型：
--
作者：
Klibanov AL;Shevchenko TI;Raju BI;Seip R;Chin CT
我们研究了基于脂质体包被的微泡悬垂复合物的超声触发药物递送系统的制备。生物素化的十氟丁烷微泡通过链霉亲和素连接体被生物素化的脂质体包被。制备掺有钙黄绿素和凝血酶的脂质体。基于钙黄绿素的初始浓度，当使用 100 nm 脂质体时，每个微泡脂质体颗粒的有效负载体积超过 1 um3。体外微泡-脂质体悬垂体的声波作用导致微泡完全破坏并引发大部分截留材料的释放。根据荧光猝灭测定的估计，用 1 MHz 超声波（5 个脉冲、100 ms、7 MPa 峰值负声压）处理导致约 30% 的包埋钙黄绿素释放。通过显色底物研究估计了由于超声处理而从与微泡（11％的截留材料）复合的脂质体中释放的凝血酶。在声波处理之前，底物水解处于背景水平。超声触发的凝血酶从悬垂复合物中释放导致血液凝固加速。
We investigated preparation of ultrasound-triggered drug delivery system, based on a pendant complex of microbubble coated with liposomes. Biotinylated decafluorobutane microbubbles were coated with biotinylated liposomes via a streptavidin linker. Liposomes were prepared incorporating calcein and thrombin. Based on initial concentration of calcein, over 1 um3 payload volume per each microbubble-liposome particle was achieved, when 100 nm liposomes were used. Insonation of microbubble-liposome pendants in vitro resulted in the complete destruction of microbubbles and triggered release of a significant fraction of the entrapped material. Treatment with 1 MHz ultrasound (5 pulses, 100 ms, 7 MPa peak negative acoustic pressure) resulted in the release of ~30% of entrapped calcein, as estimated by the fluorescence quenching assay. Thrombin release from liposomes complexed with microbubbles (11% of entrapped material) due to ultrasound treatment was estimated by a chromogenic substrate study. Prior to insonation, substrate hydrolysis was at background level. Ultrasound-triggered release of thrombin from the pendant complexes caused acceleration of blood clotting.
登录
查看更多内容
影响因子：
--
作者：
Klibanov, A. L.;Rychak, J. J.;Kaul, S.
通讯作者：
Kaul, S.
影响因子：
19.7
作者：
Yuh, EL;Shulman, SG;Li, KCP
通讯作者：
Li, KCP
影响因子：
11.5
作者：
Hauck, Marlene L.;LaRue, Susan M.;Dewhirst, Mark W.
通讯作者：
Dewhirst, Mark W.
影响因子：
4
作者：
Chomas, JE;Dayton, PA;Ferrara, K
通讯作者：
Ferrara, K
影响因子：
64.8
作者：
Marmottant, P;Hilgenfeldt, S
通讯作者：
Hilgenfeldt, S