Temporal and SUMO-specific SUMOylation contribute to the dynamics of Polo-like kinase 1 (PLK1) and spindle integrity during mouse oocyte meiosis.
Temporal and SUMO-specific SUMOylation contribute to the dynamics of Polo-like kinase 1 (PLK1) and spindle integrity during mouse oocyte meiosis.
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DOI：
10.1016/j.ydbio.2017.12.011
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发表时间：
2018-02-15
影响因子：
2.7
通讯作者：
Morris PL
中科院分区：
文献类型：
--
作者：
Feitosa WB;Hwang K;Morris PL
在哺乳动物减数分裂过程中，Polo样激酶1（PLK 1）在细胞周期进程中是必不可少的。在卵母细胞成熟过程中，PLK 1的表达已得到很好的表征，但调控其活性的翻译后修饰的时间和亚细胞定位尚不清楚。小泛素相关修饰蛋白（SUMO）翻译后修饰蛋白已在哺乳动物配子中检测到，但其在配子发生过程中的确切功能在很大程度上是未知的。在本文中，我们报告的小鼠卵母细胞PLK 1和磷酸化PLK 1进行SUMO化减数分裂II（MII）卵母细胞使用免疫细胞化学，免疫沉淀和体外SUMO化试验。在MII，PLK 1在苏氨酸-210和丝氨酸-137处磷酸化。MII卵母细胞PLK 1和磷酸化的PLK 1通过SUMO-1、-2和-3进行SUMO化，如单独的体外测定所示。使用这些测定，磷酸化PLK 1的形式标准化PLK 1显着增加，并与SUMO化PLK 1水平相关。在减数分裂进程和成熟过程中，PLK 1的SUMO-1-SUMO化参与纺锤体的形成，而SUMO-2/3-SUMO化可能调节PLK 1在着丝粒-纺锤体附着位点的活性。微管的完整性是PLK 1与SUMO-1定位所必需的，但不是与SUMO-2/3。抑制SUMO化会破坏正常的减数分裂双极纺锤体组织和纺锤体-动粒附着。这些数据表明，时间和SUMO特异性SUMO化在小鼠卵母细胞减数分裂过程中协调PLK 1的功能动力学，包括亚细胞区室化中发挥重要作用。
During mammalian meiosis, Polo-like kinase 1 (PLK1) is essential during cell cycle progression. In oocyte maturation, PLK1 expression is well characterized but timing of posttranslational modifications regulating its activity and subcellular localization are less clear. Small ubiquitin-related modifier (SUMO) posttranslational modifier proteins have been detected in mammalian gametes but their precise function during gametogenesis is largely unknown. In the present paper we report for mouse oocytes that both PLK1 and phosphorylated PLK1 undergo SUMOylation in meiosis II (MII) oocytes using immunocytochemistry, immunoprecipitation and in vitro SUMOylation assays. At MII, PLK1 is phosphorylated at threonine-210 and serine-137. MII oocyte PLK1 and phosphorylated PLK1 undergo SUMOylation by SUMO-1, -2 and -3 as shown by individual in vitro assays. Using these assays, forms of phosphorylated PLK1 normalized to PLK1 increased significantly and correlated with SUMOylated PLK1 levels. During meiotic progression and maturation, SUMO-1-SUMOylation of PLK1 is involved in spindle formation whereas SUMO-2/3-SUMOylation may regulate PLK1 activity at kinetochore-spindle attachment sites. Microtubule integrity is required for PLK1 localization with SUMO-1 but not with SUMO-2/3. Inhibition of SUMOylation disrupts proper meiotic bipolar spindle organization and spindle-kinetochore attachment. The data show that both temporal and SUMO-specific-SUMOylation play important roles in orchestrating functional dynamics of PLK1 during mouse oocyte meiosis, including subcellular compartmentalization.
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DOI：
10.1073/pnas.0701140104
发表时间：
2007-03-13
影响因子：
11.1
作者：
Burkard, Mark E.;Randall, Catherine L.;Jallepalli, Prasad V.
通讯作者：
Jallepalli, Prasad V.
DOI：
10.1073/pnas.1106223108
发表时间：
2011-07-12
影响因子：
11.1
作者：
Johmura, Yoshikazu;Soung, Nak-Kyun;Lee, Kyung S.
通讯作者：
Lee, Kyung S.
影响因子：
1.6
作者：
Gonzalez, C;Sunkel, CE;Glover, DM
通讯作者：
Glover, DM
影响因子：
7.8
作者：
Haren, Laurence;Remy, Marie-Helene;Bazin, Ingrid;Callebaut, Isabelle;Wright, Michel;Merdes, Andreas
通讯作者：
Merdes, Andreas
DOI：
10.1093/humrep/den300
发表时间：
2008-12
期刊：
Human reproduction (Oxford, England)
影响因子：
--
作者：
Brown PW;Hwang K;Schlegel PN;Morris PL
通讯作者：
Morris PL