Rapid and accurate synthesis of TALE genes from synthetic oligonucleotides.
Rapid and accurate synthesis of TALE genes from synthetic oligonucleotides.
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从合成寡核苷酸快速准确地合成 TALE 基因。
DOI：
10.2144/000114422
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发表时间：
2016-06
期刊：
影响因子：
2.7
通讯作者：
Peng Gang
中科院分区：
文献类型：
--
作者：
Wang Fenghua;Zhang Hefei;Gao Jingxia;Chen Fengjiao;Chen Sijie;Zhang Cuizhen;Peng Gang
转录激活因子样效应物（TALE）基因的定制合成依赖于预制的TALE重复单体或寡聚体的质粒文库。在此我们描述一种新的合成方法，该方法直接掺入退火的合成寡核苷酸。
Custom synthesis of transcription activator-like effector (TALE) genes has relied upon plasmid libraries of pre-fabricated TALE-repeat monomers or oligomers. Here we describe a novel synthesis method that directly incorporates annealed synthetic oligonucleotides into the TALE-repeat units. Our approach utilizes iterative sets of oligonucleotides and a translational frame check strategy to ensure the high efficiency and accuracy of TALE-gene synthesis. TALE arrays of more than 20 repeats can be constructed, and the majority of the synthesized constructs have perfect sequences. In addition, this novel oligonucleotide-based method can readily accommodate design changes to the TALE repeats. We demonstrated an increased gene targeting efficiency against a genomic site containing a potentially methylated cytosine by incorporating non-conventional repeat variable di-residue (RVD) sequences.
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影响因子：
17.3
作者：
Ma S;Saaem I;Tian J
通讯作者：
Tian J
影响因子：
4.6
作者：
Sakuma, Tetsushi;Ochiai, Hiroshi;Kaneko, Takehito;Mashimo, Tomoji;Tokumasu, Daisuke;Sakane, Yuto;Suzuki, Ken-ichi;Miyamoto, Tatsuo;Sakamoto, Naoaki;Matsuura, Shinya;Yamamoto, Takashi
通讯作者：
Yamamoto, Takashi
影响因子：
48
作者：
Schwartz, Jerrod J.;Lee, Choli;Shendure, Jay
通讯作者：
Shendure, Jay
使用非规定RVD对TALEN特异性进行优化调整。
影响因子：
4.6
作者：
Juillerat A;Pessereau C;Dubois G;Guyot V;Maréchal A;Valton J;Daboussi F;Poirot L;Duclert A;Duchateau P
通讯作者：
Duchateau P
影响因子：
14.9
作者：
Cermak T;Doyle EL;Christian M;Wang L;Zhang Y;Schmidt C;Baller JA;Somia NV;Bogdanove AJ;Voytas DF
通讯作者：
Voytas DF