Molecular characterization of two recombinant potato virus Y isolates from China

Molecular characterization of two recombinant potato virus Y isolates from China
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两种来自中国的重组马铃薯病毒 Y 分离株的分子特征

DOI:
10.1007/s00705-011-1182-x
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发表时间:
2011-12
影响因子:
2.7
通讯作者:
竺晓平
竺晓平
中科院分区:
医学4区
文献类型:
--
作者:
竺晓平

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马铃薯Y病毒(PVY)分离物已分为几个株系。我们测定了我国烟草PVY分离物AQ 4和FZ 10的基因组序列。AQ 4和FZ 10的基因组分别为9700和9698个核苷酸。在全基因组序列的系统发育分析中,AQ 4与N-Wi聚在一起,FZ 10与NTN聚在一起。AQ 4在P1和P3基因内有两个重组位点,而FZ 10在P1、P3和NIa-Pro基因内有三个重组位点。当与典型的NTN分离物相比时,FZ 10在CP基因内缺乏重组位点,因此代表了PVY的新重组类型。
Isolates of potato virus Y (PVY) have been divided into several strains. We determined the genomic sequences of PVY isolates AQ4 and FZ10 from tobacco in China. AQ4 and FZ10 had genome of 9700 and 9698 nucleotides, respectively. In phylogenetic analysis of complete genome sequences, AQ4 was clustered with strain N-Wi, and FZ10 with NTN. AQ4 had two recombination sites within the P1 and P3 genes, while FZ10 had three within the P1, P3 and NIa-Pro genes. When compared to typical NTN isolates, FZ10 lacked a recombination site within the CP gene and, thus, represents a novel recombination type of PVY.
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