RNA analysis of B cell lines arrested at defined stages of differentiation allows for an approximation of gene expression patterns during B cell development
RNA analysis of B cell lines arrested at defined stages of differentiation allows for an approximation of gene expression patterns during B cell development
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对在特定分化阶段停滞的 B 细胞系进行 RNA 分析，可以近似了解 B 细胞发育过程中的基因表达模式
DOI：
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发表时间：
2003
影响因子：
5.5
通讯作者：
M. Sigvardsson
中科院分区：
文献类型：
--
作者：
P. Tsapogas;T. Breslin;S. Bilke;Anna Lagergren;R. Månsson;D. Liberg;C. Peterson;M. Sigvardsson
从骨髓干细胞发育成成熟的B淋巴细胞是一个高度有序的过程，涉及具有确定特征和基因表达模式的阶段。为了更深入地了解这一过程的分子遗传学，我们使用Affytron ™微阵列对一组代表B细胞发育特定阶段的小鼠B谱系细胞系进行了RNA表达分析。根据细胞先前定义的表型特征对细胞进行分组，并建立每组细胞系的基因表达模式。数据表明，代表确定阶段的细胞系通常在总体表达谱中呈现出高度相似性。使用基于dCHIP的定量比较或基于存在/不存在的概率状态分析，可以将许多基因鉴定为以受限模式表达。这些实验为B细胞发育过程中的基因表达提供了一个模型，并且正确鉴定的许多控制基因的表达模式表明，一系列细胞系可以是阐明复杂分化过程的分子遗传学的有用工具。
The development of a mature B lymphocyte from a bone marrow stem cell is a highly ordered process involving stages with defined features and gene expression patterns. To obtain a deeper understanding of the molecular genetics of this process, we have performed RNA expression analysis of a set of mouse B lineage cell lines representing defined stages of B cell development using Affymetrix™ microarrays. The cells were grouped based on their previously defined phenotypic features, and a gene expression pattern for each group of cell lines was established. The data indicated that the cell lines representing a defined stage generally presented a high similarity in overall expression profiles. Numerous genes could be identified as expressed with a restricted pattern using dCHIP‐based, quantitative comparisons or presence/absence‐based, probabilistic state analysis. These experiments provide a model for gene expression during B cell development, and the correctly identified expression patterns of a number of control genes suggest that a series of cell lines can be useful tools in the elucidation of the molecular genetics of a complex differentiation process.
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影响因子：
32.4
作者：
Fu, C;Turck, CW;Chan, AC
通讯作者：
Chan, AC
影响因子：
32.4
作者：
Li, YS;Wasserman, R;Hardy, RR
通讯作者：
Hardy, RR
通过耐受敏感的未成熟鼠 B 淋巴细胞表面 IgM 发出信号。
DOI：
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发表时间：
1991
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Yellen,AJ;Glenn,W;Sukhatme,VP;Cao,XM;Monroe,JG
通讯作者：
Monroe,JG
影响因子：
32.4
作者：
Tudor, KSRS;Payne, KJ;Kincade, PW
通讯作者：
Kincade, PW
影响因子：
20.3
作者：
CATTORETTI, G;CHANG, CC;DALLAFAVERA, R
通讯作者：
DALLAFAVERA, R