Chondrogenic Potency Analyses of Donor-Matched Chondrocytes and Mesenchymal Stem Cells Derived from Bone Marrow, Infrapatellar Fat Pad, and Subcutaneous Fat.
Chondrogenic Potency Analyses of Donor-Matched Chondrocytes and Mesenchymal Stem Cells Derived from Bone Marrow, Infrapatellar Fat Pad, and Subcutaneous Fat.
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DOI：
10.1155/2016/6969726
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发表时间：
2016
影响因子：
4.3
通讯作者：
Wright KT
中科院分区：
文献类型：
--
作者：
Garcia J;Mennan C;McCarthy HS;Roberts S;Richardson JB;Wright KT
自体软骨细胞植入（ACI）是一种基于细胞的治疗方法，已经在临床上使用了20多年，用于更有效地治疗软骨损伤，以消除或延迟关节置换手术的需要。在这段时间里，ACI程序几乎没有改变，但现在许多中心正在考虑或使用替代细胞来源进行软骨修复，特别是间充质干细胞（MSCs）。在这项研究中，我们测试了来自骨髓（BM）、髌下脂肪垫（FP）和皮下脂肪（SCF）的供体匹配的间充质干细胞与软骨细胞的成软骨潜能。我们已经证实，在这些细胞类型中存在成软骨能力等级，最强大的是软骨细胞，其次是FP-MSCs， BM-MSCs，最后是SCF-MSCs。我们还在预测模型中检查了基因表达和表面标记谱，以鉴定具有增强软骨形成潜力的细胞。在此过程中，我们已经表明，Sox-9、Alk-1和Coll X的表达，以及CD49c和CD39的免疫阳性，对所有类型的细胞都具有预测成软骨潜能的价值。本研究结果对改进和发展新的细胞软骨修复策略具有重要的临床意义。
Autologous chondrocyte implantation (ACI) is a cell-based therapy that has been used clinically for over 20 years to treat cartilage injuries more efficiently in order to negate or delay the need for joint replacement surgery. In this time, very little has changed in the ACI procedure, but now many centres are considering or using alternative cell sources for cartilage repair, in particular mesenchymal stem cells (MSCs). In this study, we have tested the chondrogenic potential of donor-matched MSCs derived from bone marrow (BM), infrapatellar fat pad (FP), and subcutaneous fat (SCF), compared to chondrocytes. We have confirmed that there is a chondrogenic potency hierarchy ranging across these cell types, with the most potent being chondrocytes, followed by FP-MSCs, BM-MSCs, and lastly SCF-MSCs. We have also examined gene expression and surface marker profiles in a predictive model to identify cells with enhanced chondrogenic potential. In doing so, we have shown that Sox-9, Alk-1, and Coll X expressions, as well as immunopositivity for CD49c and CD39, have predictive value for all of the cell types tested in indicating chondrogenic potency. The findings from this study have significant clinical implications for the refinement and development of novel cell-based cartilage repair strategies.
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影响因子：
7.4
作者：
Choudhery MS;Badowski M;Muise A;Pierce J;Harris DT
通讯作者：
Harris DT
影响因子：
2.8
作者：
McCarthy HS;Richardson JB;Parker JC;Roberts S
通讯作者：
Roberts S
影响因子：
5.5
作者：
Gullo, Francesca;De Bari, Cosimo
通讯作者：
De Bari, Cosimo
DOI：
10.1073/pnas.46.12.1533
发表时间：
1960-01-01
影响因子：
11.1
作者：
HOLTZER, H;ABBOTT, J;HOLTZER, S
通讯作者：
HOLTZER, S
DOI：
10.1016/0304-4165(86)90306-5
发表时间：
1986-09-04
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
FARNDALE, RW;BUTTLE, DJ;BARRETT, AJ
通讯作者：
BARRETT, AJ