Donor age negatively impacts adipose tissue-derived mesenchymal stem cell expansion and differentiation.
Donor age negatively impacts adipose tissue-derived mesenchymal stem cell expansion and differentiation.
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DOI：
10.1186/1479-5876-12-8
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发表时间：
2014-01-07
影响因子：
7.4
通讯作者：
Harris DT
中科院分区：
文献类型：
--
作者：
Choudhery MS;Badowski M;Muise A;Pierce J;Harris DT
人脂肪组织是一种理想的自体间充质干细胞（MSCs）来源，用于各种再生医学和组织工程策略。老年患者是许多有前途的应用的主要目标人群之一。人们早就知道，高龄与生物体的修复和再生潜力呈负相关，但关于年龄对人脂肪组织来源的MSC（hAT-MSC）质量的影响的信息很少且相互矛盾。为了研究年龄的影响，研究了来自年轻（<30岁）、成年（35-50岁）和老年（>60岁）个体的hAT-MSC的扩增和体外分化潜力。MSC的特点是表达基因p16 INK 4a和p21沿着与测量群体倍增（PD），超氧化物歧化酶（SOD）活性，细胞衰老和分化潜力。与从年轻供体分离的细胞相比，老年MSC显示衰老特征，伴随着活力和增殖降低。与年轻MSC相比，这些特征也与老年MSC中显著降低的分化潜力相关。总之，年龄的增长对干细胞功能产生负面影响，这种与年龄相关的变化可能对成功的干细胞疗法不利。
Human adipose tissue is an ideal autologous source of mesenchymal stem cells (MSCs) for various regenerative medicine and tissue engineering strategies. Aged patients are one of the primary target populations for many promising applications. It has long been known that advanced age is negatively correlated with an organism’s reparative and regenerative potential, but little and conflicting information is available about the effects of age on the quality of human adipose tissue derived MSCs (hAT-MSCs). To study the influence of age, the expansion and in vitro differentiation potential of hAT-MSCs from young (<30 years), adult (35-50 years) and aged (>60 years) individuals were investigated. MSCs were characterized for expression of the genes p16INK4a and p21 along with measurements of population doublings (PD), superoxide dismutase (SOD) activity, cellular senescence and differentiation potential. Aged MSCs displayed senescent features when compared with cells isolated from young donors, concomitant with reduced viability and proliferation. These features were also associated with significantly reduced differentiation potential in aged MSCs compared to young MSCs. In conclusion, advancing age negatively impacts stem cell function and such age related alterations may be detrimental for successful stem cell therapies.
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通讯作者：
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