Long non-coding RNA PGM5-AS1 promotes epithelial-mesenchymal transition, invasion and metastasis of osteosarcoma cells by impairing miR-140-5p-mediated FBN1 inhibition.
Long non-coding RNA PGM5-AS1 promotes epithelial-mesenchymal transition, invasion and metastasis of osteosarcoma cells by impairing miR-140-5p-mediated FBN1 inhibition.
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长的非编码RNA PGM5-AS1通过损害miR-140-5p介导的FBN1抑制，促进骨肉瘤细胞的上皮 - 间质转变，侵袭和转移。
DOI：
10.1002/1878-0261.12711
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发表时间：
2020-10
影响因子：
6.6
通讯作者：
Yan M
中科院分区：
文献类型：
--
作者：
Liu W;Liu P;Gao H;Wang X;Yan M
Osteosarcoma is an uncommon tumor occurring in bone, accompanied by elevated incidence and reduced rate of healing. Epithelial‐to‐mesenchymal transition (EMT) serves as a conceptual paradigm to explain the invasion and metastasis of osteosarcoma and other cancers. Hence, developing effective therapeutic strategy to treat the EMT of osteosarcoma is essential. Here, we identified the molecular mechanism of long noncoding RNA (lncRNA) PGM5‐AS1 in EMT and progression of osteosarcoma. Microarray‐based analysis was employed to screen the osteosarcoma‐related differentially expressed lncRNAs. The levels of PGM5‐AS1 as well as microRNA‐140‐5p (miR‐140‐5p) and fibrillin‐1 (FBN1) in osteosarcoma tissues and cells were determined. Dual‐luciferase reporter gene assay, RNA pull‐down assay, and RNA immunoprecipitation assay were conducted to validate the relationship among PGM5‐AS1, miR‐140‐5p, and FBN1. Expression of PGM5‐AS1, miR‐140‐5p, and FBN1 was altered by overexpression, shRNA, mimic, or inhibitors in order to investigate how they regulated migration, invasion, and EMT of osteosarcoma cells in vitro. Loss‐ and gain‐of‐function approaches were employed in nude mice to detect their roles in tumorigenesis in vivo. Osteosarcoma tissues and cells exhibited low expression of miR‐140‐5p, but high expression of PGM5‐AS1 and FBN1. PGM5‐AS1 competitively bound to miR‐140‐5p to upregulate FBN1. Furthermore, hindering PGM5‐AS1 and FBN1 or overexpressing miR‐140‐5p dampened migration, invasion, and EMT of osteosarcoma cells in vitro. Furthermore, silencing PGM5‐AS1 or FBN1, or overexpressing miR‐140‐5p markedly inhibited tumorigenesis in nude mice in vivo. Taken together, PGM5‐AS1 depletion causes FBN1 reduction to retard osteosarcoma processes by negatively modulating miR‐140‐5p. In this study, we analyzed the modulatory role of PGM5‐AS1 in the progression and EMT of osteosarcoma. In osteosarcoma tissues and cells, PGM5‐AS1 and FBN1 are highly expressed, while miR‐140‐5p exhibits low expression. We reveal that PGM5‐AS1 specifically binds to miR‐140‐5p to silence the expression of FBN1, and thereby attenuate EMT, invasion, and migration of osteosarcoma in vitro as well as tumorigenesis in vivo. In this study, we analyzed the modulatory role of PGM5‐AS1 in the progression and EMT of osteosarcoma. In osteosarcoma tissues and cells, PGM5‐AS1 and FBN1 are highly expressed, while miR‐140‐5p exhibits low expression. We reveal that PGM5‐AS1 specifically binds to miR‐140‐5p to silence the expression of FBN1, and thereby attenuate EMT, invasion, and migration of osteosarcoma in vitro as well as tumorigenesis in vivo.
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影响因子：
2.1
作者：
Güllü G;Peker I;Haholu A;Eren F;Küçükodaci Z;Güleç B;Baloglu H;Erzik C;Özer A;Akkiprik M
通讯作者：
Akkiprik M
捕获microRNA结合的mRNA将肿瘤抑制器miR-34a识别为生长因子信号传导的调节剂。
影响因子：
4.5
作者：
Lal A;Thomas MP;Altschuler G;Navarro F;O'Day E;Li XL;Concepcion C;Han YC;Thiery J;Rajani DK;Deutsch A;Hofmann O;Ventura A;Hide W;Lieberman J
通讯作者：
Lieberman J
影响因子：
1.2
作者：
Bozycki, Lukasz;Komiazyk, Magdalena;Strzelecka-Kiliszek, Agnieszka
通讯作者：
Strzelecka-Kiliszek, Agnieszka
影响因子：
--
作者：
Han JA;Kim JY;Kim JI
通讯作者：
Kim JI
影响因子：
7.5
作者：
Lan, Hong;Chen, Wangsheng;Yang, Shuying
通讯作者：
Yang, Shuying