In vitro investigation of chemical properties and biocompatibility of neurovascular braided implants.
In vitro investigation of chemical properties and biocompatibility of neurovascular braided implants.
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DOI：
10.1007/s10856-019-6270-6
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发表时间：
2019-06-04
期刊：
影响因子：
--
通讯作者：
Krajewski S
中科院分区：
文献类型：
--
作者：
Cattaneo G;Bräuner C;Siekmeyer G;Ding A;Bauer S;Wohlschlögel M;Lang L;Hierlemann T;Akimov M;Schlensak C;Schüßler A;Wendel HP;Krajewski S
镍钛合金微导丝编织是一种成熟的技术，用于制造用于迂曲血管几何形状的细网神经血管植入物。在编织过程之前对金属丝进行电抛光有可能改善血栓形成和内皮细胞增殖方面的体外行为。在本研究中，我们首次对血流环路中的编织电解抛光/蓝色氧化镍钛合金样本进行了体外研究，结果显示凝血途径激活显著降低（由达特III标记物表示），血小板粘附倾向降低。此外，我们对扁平圆盘进行了相同的表面处理，并测量了蛋白质粘附和内皮细胞增殖。我们将我们的结果与具有天然氧化物表面的非电抛光样品进行了比较。虽然电抛光/蓝色氧化物表面上的血小板沉积减少，但观察到内皮细胞接种显著增加。内皮细胞中炎症标志物表达的研究提供了不同的结果，这取决于测试的标志物，需要更深入的研究。使用俄歇电子能谱进行的表面分析显示，主要由氮氧化钛或氧化钛+氮化钛组成的薄层是生物性能改善的潜在原因。转化为颅内动脉瘤治疗的临床领域，生物相容性的改善有可能提高安全性（低血栓形成性）和有效性（动脉瘤颈重建）。
Braiding of Nitinol micro wires is an established technology for the manufacturing of fine-meshed neurovascular implants for tortuous vessel geometries. Electropolishing of wires before the braiding process has the potential to improve the in vitro behaviour in terms of thrombogenicity and endothelial cell proliferation. In this study, we present the first in vitro investigation of braided electropolished/blue oxide Nitinol samples in a blood flow loop, showing a significantly lower activation of the coagulation pathway (represented by the TAT III marker) and a tendency towards reduced platelet adhesion. Furthermore, we applied the same surface treatment on flat disks and measured protein adhesion as well as endothelial cell proliferation. We compared our results to non-electropolished samples with a native oxide surface. While platelet deposition was reduced on electropolished/blue oxide surface, a significant increase of endothelial cell seeding was observed. Investigation of inflammatory marker expression in endothelial cells provided divergent results depending on the marker tested, demanding closer investigation. Surface analysis using Auger electron spectroscopy revealed a thin layer mainly consisting of titanium oxynitride or titanium oxide + titanium nitride as a potential cause of the improved biological performance. Translated to the clinical field of intracranial aneurysm treatment, the improved biocompatibility has the potential to increase both safety (low thrombogenicity) and effectiveness (aneurysm neck reconstruction).
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