Soil and Leaf Nutrients Drivers on the Chemical Composition of the Essential Oil of Siparuna muricata (Ruiz & Pav.) A. DC. from Ecuador.
Soil and Leaf Nutrients Drivers on the Chemical Composition of the Essential Oil of Siparuna muricata (Ruiz & Pav.) A. DC. from Ecuador.
复制标题
DOI：
10.3390/molecules26102949
复制
发表时间：
2021-05-15
期刊：
影响因子：
--
通讯作者：
Morocho V
中科院分区：
文献类型：
--
作者：
Burneo JI;Benítez Á;Calva J;Velastegui P;Morocho V
植物的化学成分受土壤中初始养分含量和气候条件的影响，因此，我们首次分析了土壤和叶片养分对厄瓜多尔四个不同地区的西帕鲁纳植物精油(Eos)组成的影响。采用水蒸气蒸馏法提取环氧乙烷，用气相色谱/质谱仪(GC/MS)和气相色谱/火焰离子化检测器(GC/FID)对其进行分析。用GC/MS测定了对映体的分布，改进了β-品烯、柠檬烯、δ-榄香烯、β-波波烯、顺式-1(6)、4-二烯和苍术酮的对映体分离。共鉴定出44个化合物。L1最具代表性的是愈创木酚、苍术酮和4-二烯；L2最具代表性的是顺式-1(6)、4-二烯和月桂烯；L3最具代表性的是苍术酮、月桂烯和香叶烯B；最后是L4香叶烯B、月桂烯和顺式-1(6)、4-二烯。不同产地土壤-叶片中的铝、钙、铁、镁、锰、氮、硅等化学元素与西百合挥发油化学成分的相关性显著，而土壤与叶片中的钾、磷、钠相关性不显著。聚类分析和NMDS分析表明，4个地点次生代谢物的差异值较高，与土壤-叶片养分的变化有关。因此，SIMER例程显示，并不是所有的次生代谢物对确定四个地点的差异都有同等的贡献，最大的贡献是由于愈创木酚、顺式-1(6)、4-二烯、苍术酮和杰马林的差异。本研究首次揭示了海拔和土壤-叶片化学元素对沙打旺Eos化学成分的影响。
Chemical compositions of plants are affected by the initial nutrient contents in the soil and climatic conditions; thus, we analyzed for the first time the effects of soil and leaf nutrients on the compositions of the essential oils (EOs) of Siparuna muricata in four different localities in Ecuador. EOs were obtained by hydrodistillation and analyzed by gas chromatography/mass spectrometry (GC/MS) and a gas chromatography/flame ionization detector (GC/FID). Enantiomeric distribution by GC/MS was determined, modifying the enantiomeric separation of β-pinene, limonene, δ-elemene, β-bourbonene, cis-cadina-1 (6), 4-diene and atractylone. A total of 44 compounds were identified. The most representative for L1 were guaiol, atractylone and 4-diene; for L2, cis-cadina-1(6),4-diene and myrcene; for L3, atractylone, myrcene and germacrene B; and finally, L4 germacrene B, myrcene and cis-cadina-1(6),4-diene. Correlations between soil- leaf chemical elements such as Al, Ca, Fe, Mg, Mn, N and Si in the different localities were significant with chemical composition of the essential oil of Siparuna muricata; however, correlations between soil and leaf K, P, and Na were not significant. Cluster and NMDS analysis showed high dissimilarity values of secondary metabolites between four localities related with changes in soil- leaf nutrients. Thus, the SIMPER routine revealed that not all secondary metabolites contribute equally to establishing the differences in the four localities, and the largest contributions are due to differences in guaiol, cis-cadina-1(6),4-diene, atractylone and germacrene. Our investigation showed for the first time the influences of altitude and soil- leaf chemical elements in the chemical composition of the EOs of S. muricata.
登录
查看更多内容
影响因子：
4.5
作者：
Garcia, Jessica;Gilardoni, Gianluca;Morocho, Vladimir
通讯作者：
Morocho, Vladimir
影响因子：
--
作者：
BRAY, RH;KURTZ, LT
通讯作者：
KURTZ, LT
影响因子：
2.3
作者：
Amzallag, GN;Larkov, O;Dudai, N
通讯作者：
Dudai, N
影响因子：
1.9
作者：
Calva, James;Castillo, Jessica M.;Armijos, Chabaco
通讯作者：
Armijos, Chabaco
影响因子：
3.6
作者：
Kumari, Manglesh;Joshi, Robin;Kumar, Rajiv
通讯作者：
Kumar, Rajiv