Aggregation formation mediated anoikis resistance of BEL7402 hepatoma cells.
Aggregation formation mediated anoikis resistance of BEL7402 hepatoma cells.
复制标题
聚集形成介导 BEL7402 肝癌细胞的失巢凋亡抵抗。
DOI：
10.2478/v10042-008-0042-3
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发表时间：
2008
期刊：
影响因子：
--
通讯作者：
L Cao
中科院分区：
文献类型：
--
作者：
X Liang;Z Qu;H Liu;Z Zhang;Y Liu;L Han;J Du;L Cao
失巢诱导耐药是癌细胞转移的前提。阐明失巢细胞抵抗的机制仍然是一个重大的挑战。我们报道了一种体外模拟肝癌细胞失巢凋亡耐药过程的模型。实验结果表明，细胞间聚集可介导BEL7402肝癌细胞对失巢凋亡的抵抗。对这些聚集体的进一步研究表明，在肝癌细胞失去锚定后，其生物学特性发生了很大的变化。聚集体的形成过程根据其生物学特性可分为三个不同的阶段，即成熟期、成熟期和后成熟期。成熟时相聚集体具有细胞活性的高级状态，并可能模仿循环系统中的转移细胞。对这三种相聚集体的生物学特性进行了详细的研究，包括形态变化、细胞活力和微阵列表达谱。提示在聚集形成过程中，黏附分子大量上调，聚集过程中细胞间的接触可能不依赖于钙离子参与的黏附途径。该模型可能有助于阐明肝癌细胞的失巢凋亡耐药机制，并有助于开发针对抗失巢凋亡肝癌细胞在转移过程中的新的治疗方法。
Anoikis resistance is the prerequisite of cancer cells metastasis. Elucidation of the mechanism of anoikis resistance remains a significant challenge. We reported here a model to mimic anoikis resistant process of hepatoma cells in vitro. Experimental results indicated cell to cell aggregation could mediate anoikis resistance of BEL7402 hepatoma cells. Further investigation of these aggregations indicated the biological properties changed greatly after the hepatoma cells lost their anchorage. Aggregation forming process could be separated into three distinct phases according to their biological characteristics, comprising of premature phase, mature phase and postmature phase. Mature phase aggregations have the premium state of cell viability and may mimic the metastatic cells in the circulating system. Biological properties of these three phases aggregations were studied in details including morphological alteration, cell viability and microarray expression profiles. It indicated there was a great upregulation of adhesion molecules during the process of aggregation formation and the cell to cell contact in the aggregation may be mediated independent of calcium involved adhesion pathway. This model might shed light on the anoikis resistance mechanism of hepatoma cells and help to develop new therapies that may target the anoikis resistant hepatoma cells in the metastasis process.
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影响因子：
4.2
作者：
D. Ma;F. Nothias;L. Boyne;I. Fischer
通讯作者：
D. Ma;F. Nothias;L. Boyne;I. Fischer
DOI：
--
发表时间：
2004
期刊：
Journal de la Societe de biologie
影响因子：
--
作者：
Stéphane Fouquet;Verónica-Haydée Lugo-Martínez;J. Chambaz;P. Cardot;M. Pinçon-Raymond;S. Thenet
通讯作者：
Stéphane Fouquet;Verónica-Haydée Lugo-Martínez;J. Chambaz;P. Cardot;M. Pinçon-Raymond;S. Thenet
影响因子：
2.7
作者：
K. O'Connor;M. Venczel
通讯作者：
K. O'Connor;M. Venczel
影响因子：
--
作者：
Reddy, P;Liu, LA;Liu, K
通讯作者：
Liu, K
影响因子：
6
作者：
Shen, XD;Kramer, RH
通讯作者：
Kramer, RH