Alternative preparation of propolis extracts: comparison of their composition and biological activities.
Alternative preparation of propolis extracts: comparison of their composition and biological activities.
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DOI：
10.1186/s12906-015-0677-5
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发表时间：
2015-05-27
影响因子：
--
通讯作者：
Savickas A
中科院分区：
文献类型：
--
作者：
Kubiliene L;Laugaliene V;Pavilonis A;Maruska A;Majiene D;Barcauskaite K;Kubilius R;Kasparaviciene G;Savickas A
蜂胶是一种因具有多种生物效应而闻名的蜂产品，因此它被广泛用于多种疾病的预防和治疗。蜂胶的活性物质易溶于乙醇。然而，乙醇提取物不能用于治疗眼科、儿科等遇到的某些疾病。不幸的是，蜂胶的主要生物活性物质几乎不溶于水、油以及制药工业中常用的其他溶剂。本研究的目的是研究在非乙醇溶剂中不同方法制备的蜂胶提取物的化学成分、自由基清除能力和抗菌活性。
采用福林 - 酚试剂法测定提取物中酚类化合物的总含量。利用带有自由基反应检测器的高效液相色谱系统测定提取物的化学成分和自由基清除活性。采用琼脂孔扩散法评估所检测制剂的抗菌活性。
在聚乙二醇400（PEG）和水的混合物中，或者在PEG、橄榄油和水的混合物中于70°C制备的提取物中酚类化合物的总量与乙醇提取物相当。主要鉴定出的化合物是酚酸，其约占总自由基清除活性的40%。
所研究的非乙醇提取物抑制了所有受试微生物的生长和繁殖。一些提取物的抗菌活性等于或超过了乙醇提取物的抗菌效果。仅在室温下在纯水或油中制备的提取物所含酚类化合物的量低5 - 10倍以上，并且没有表现出抗菌活性。
非乙醇溶剂复合物以及较高温度的作用使得能够更有效地从蜂胶中提取活性化合物。这些提取物中总酚类化合物的浓度与乙醇提取物中的浓度没有显著差异。蜂胶非乙醇提取物具有自由基清除和抗菌活性。
本文的网络版（doi:10.1186/s12906 - 015 - 0677 - 5）包含补充材料，可供授权用户使用。
Propolis is the bee product noted for multiple biological effects, and therefore it is widely used for the prevention and treatment of a variety of diseases. The active substances of propolis are easily soluble in ethanol. However ethanolic extracts cannot be used in treatment of certain diseases encountered in ophthalmology, pediatrics, etc. Unfortunately, the main biologically active substances of propolis are scarcely soluble in water, oil and other solvents usually used in pharmaceutical industry. The aim of this study was to investigate chemical composition, radical scavenging and antimicrobial activity of propolis extracts differently made in nonethanolic solvents. Total content of phenolic compounds in extracts was determined using Folin-Ciocalteu method. Chemical composition and radical scavenging activity of extracts were determined using HPLC system with free radical reaction detector. Antimicrobial activity of examined preparations was evaluated using the agar-well diffusion assay. Total amount of phenolic compounds in extracts made in polyethylene glycol 400 (PEG) and water mixture or in PEG, olive oil and water mixture at 70 °C was comparable to that of ethanolic extract. Predominantly identified compounds were phenolic acids, which contribute ca. 40 % of total radical scavenging activity. Investigated nonethanolic extracts inhibited the growth and reproduction of all tested microrganisms. Antimicrobial activity of some extracts was equal or exceeded the antimicrobial effect of ethanolic extract. Extracts made in pure water or oil only at room temperature, contained more than 5 – 10-fold lower amount of phenolic compounds, and demonstrated no antimicrobial activity. Nonethanolic solvent complex and the effect of higher temperature allows more effective extraction of active compounds from propolis. Concentration of total phenolic compounds in these extracts does not differ significantly from the concentration found in ethanolic extract. Propolis nonethanolic extracts have radical scavenging and antimicrobial activity. The online version of this article (doi:10.1186/s12906-015-0677-5) contains supplementary material, which is available to authorized users.
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影响因子：
5.4
作者：
Orsolic, N;Basic, I
通讯作者：
Basic, I
影响因子：
3.8
作者：
Jorge, R.;Furtado, N. A. J. C.;Silva, M. L. A.
通讯作者：
Silva, M. L. A.
影响因子：
2.6
作者：
Ivanauskas, Liudas;Jakstas, Valdas;Baranauskas, Algirdas
通讯作者：
Baranauskas, Algirdas
影响因子：
2.2
作者：
Ramanauskiene, K.;Inkeniene, A. M.
通讯作者：
Inkeniene, A. M.
影响因子：
4.3
作者：
Valadares, B. L. B.;Graf, U.;Spano, M. A.
通讯作者：
Spano, M. A.