Modeling mammalian spermatogonial differentiation and meiotic initiation in vitro.
Modeling mammalian spermatogonial differentiation and meiotic initiation in vitro.
复制标题
DOI：
10.1242/dev.200713
复制
发表时间：
2022-11-15
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
--
作者：
在哺乳动物睾丸中，减数分裂前精原细胞对视黄酸的反应是在减数分裂开始前完成一个必要的漫长的分化程序。指导这些发育过程的分子和细胞变化在很大程度上仍不明确。这种知识上的巨大差距是由于两个尚未解决的技术挑战：（1）缺乏稳健和可靠的体外模型来研究分化和减数分裂起始;（2）缺乏在分化的每个阶段和减数分裂起始分离大而纯的雄性生殖细胞群体的方法。在这里，我们报告了一个简单的体外分化和减数分裂起始系统，可以很容易地操作，包括使用化学试剂，不能安全地管理到活的动物。此外，我们提出了一种转基因小鼠模型，使荧光激活细胞分选为基础的隔离数百万的精原细胞在特定的发育阶段，以及减数分裂精母细胞。总结：在这里，我们报告了强大而可靠的工具来模拟哺乳动物减数分裂的启动在体外和分离的纯群体的数百万细胞经历的有丝分裂到减数分裂的生化测定。
In mammalian testes, premeiotic spermatogonia respond to retinoic acid by completing an essential lengthy differentiation program before initiating meiosis. The molecular and cellular changes directing these developmental processes remain largely undefined. This wide gap in knowledge is due to two unresolved technical challenges: (1) lack of robust and reliable in vitro models to study differentiation and meiotic initiation; and (2) lack of methods to isolate large and pure populations of male germ cells at each stage of differentiation and at meiotic initiation. Here, we report a facile in vitro differentiation and meiotic initiation system that can be readily manipulated, including the use of chemical agents that cannot be safely administered to live animals. In addition, we present a transgenic mouse model enabling fluorescence-activated cell sorting-based isolation of millions of spermatogonia at specific developmental stages as well as meiotic spermatocytes. Summary: Here, we report robust and reliable tools to model mammalian meiotic initiation in vitro and isolate pure populations of millions of cells undergoing the mitosis-to-meiosis transition for biochemical assays.
登录
查看更多内容
影响因子：
2.7
作者：
Busada, Jonathan T.;Chappell, Vesna A.;Niedenberger, Bryan A.;Kaye, Evelyn P.;Keiper, Brett D.;Hogarth, Cathryn A.;Geyer, Christopher B.
通讯作者：
Geyer, Christopher B.
影响因子：
3.6
作者：
Griswold MD
通讯作者：
Griswold MD
影响因子：
14.9
作者：
Deutsch EW;Csordas A;Sun Z;Jarnuczak A;Perez-Riverol Y;Ternent T;Campbell DS;Bernal-Llinares M;Okuda S;Kawano S;Moritz RL;Carver JJ;Wang M;Ishihama Y;Bandeira N;Hermjakob H;Vizcaíno JA
通讯作者：
Vizcaíno JA
影响因子：
3.7
作者：
Genc, Baris;Lagrimas, Amiko Krisa Bunag;Oezdinler, P. Hande
通讯作者：
Oezdinler, P. Hande
小鼠睾丸中ID4+种系干细胞群的功能和分子特征。
影响因子：
10.5
作者：
Chan F;Oatley MJ;Kaucher AV;Yang QE;Bieberich CJ;Shashikant CS;Oatley JM
通讯作者：
Oatley JM