Functional and molecular features of the Id4+ germline stem cell population in mouse testes.
Functional and molecular features of the Id4+ germline stem cell population in mouse testes.
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小鼠睾丸中ID4+种系干细胞群的功能和分子特征。
DOI：
10.1101/gad.240465.114
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发表时间：
2014-06-15
影响因子：
10.5
通讯作者：
Oatley JM
中科院分区：
文献类型：
--
作者：
Chan F;Oatley MJ;Kaucher AV;Yang QE;Bieberich CJ;Shashikant CS;Oatley JM
Chan等人产生了精原干细胞表达Id 4-Gfp转基因的转基因小鼠。Id 4-Gfp+细胞主要作为A型单细胞库的一个亚群存在，最终占成年期未分化精原细胞群的1.2%。RNA测序分析揭示了Id 4-Gfp+/干细胞和Id 4-Gfp−/祖细胞组分表达独特的基因。这些发现提供了干细胞作为单个池的罕见子集存在的证据，并揭示了哺乳动物雄性生殖系中区分干细胞和祖细胞状态的转录组特征。维持循环细胞谱系依赖于由干细胞和祖细胞库组成的未分化亚群。描述这些细胞类型的特征对于许多谱系是不确定的，包括精子发生，其由未分化的精原细胞群体支持。在这里，我们产生了一个转基因小鼠系，其中精原干细胞的分化抑制剂4（Id 4）-绿色荧光蛋白（GFP）转基因的表达标记。我们发现Id 4-GFP+细胞主要作为A型单细胞库的一个子集存在，它们的频率在新生儿发育中最高，然后在生精谱系建立期间比例下降，最终占成年期未分化精原细胞群的1.2%。RNA测序分析显示，Id 4-Gfp+/干细胞和Id 4-Gfp−/祖细胞组分分别有11个和25个基因的表达是独特的。总的来说，这些发现提供了第一个明确的证据，即干细胞作为一个罕见的子集存在的单一池，并揭示转录组特征区分干细胞和祖细胞状态的哺乳动物雄性生殖系。
Chan et al. generated transgenic mice in which spermatogonial stem cells expressed an Id4-Gfp transgene. Id4-Gfp+ cells exist primarily as a subset of the type Asingle pool and eventually comprise ∼2% of the undifferentiated spermatogonial population in adulthood. RNA sequencing analysis revealed genes whose expression is unique for the Id4-Gfp+/stem cell and Id4-Gfp−/progenitor fractions. These findings provide evidence that stem cells exist as a rare subset of the Asingle pool and reveal transcriptome features distinguishing stem cell and progenitor states within the mammalian male germline. The maintenance of cycling cell lineages relies on undifferentiated subpopulations consisting of stem and progenitor pools. Features that delineate these cell types are undefined for many lineages, including spermatogenesis, which is supported by an undifferentiated spermatogonial population. Here, we generated a transgenic mouse line in which spermatogonial stem cells are marked by expression of an inhibitor of differentiation 4 (Id4)-green fluorescent protein (Gfp) transgene. We found that Id4-Gfp+ cells exist primarily as a subset of the type Asingle pool, and their frequency is greatest in neonatal development and then decreases in proportion during establishment of the spermatogenic lineage, eventually comprising ∼2% of the undifferentiated spermatogonial population in adulthood. RNA sequencing analysis revealed that expression of 11 and 25 genes is unique for the Id4-Gfp+/stem cell and Id4-Gfp−/progenitor fractions, respectively. Collectively, these findings provide the first definitive evidence that stem cells exist as a rare subset of the Asingle pool and reveal transcriptome features distinguishing stem cell and progenitor states within the mammalian male germline.
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影响因子：
3.6
作者：
Nagano, MC
通讯作者：
Nagano, MC
影响因子：
3.3
作者：
Du, Y.;Yip, H. K.
通讯作者：
Yip, H. K.
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作者：
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作者：
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通讯作者：
Hochedlinger, Konrad
影响因子：
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作者：
Kuijk EW;de Gier J;Lopes SM;Chambers I;van Pelt AM;Colenbrander B;Roelen BA
通讯作者：
Roelen BA