Cognitive and Cellular Effects of Combined Organophosphate Toxicity and Mild Traumatic Brain Injury.
Cognitive and Cellular Effects of Combined Organophosphate Toxicity and Mild Traumatic Brain Injury.
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组合有机磷酸盐毒性和轻度创伤性脑损伤的认知和细胞作用。
DOI：
10.3390/biomedicines11051481
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发表时间：
2023-05-19
期刊：
影响因子：
4.7
通讯作者：
中科院分区：
文献类型：
--
作者：
创伤性脑损伤（TBI）被认为是50岁以下人群中最常见的神经系统疾病。在现代战区，TBI和有机磷（OP）的组合可能对大脑造成致命和长期的影响。我们利用小鼠闭头TBI模型诱导的重量下降装置，沿着OP暴露于对氧磷。空间和视觉记忆，以及神经元损失和反应性星形胶质细胞增生暴露于轻度TBI（mTBI）和/或对氧磷后30天进行测量。评估了颞叶皮质和海马的分子和细胞变化。认知和行为缺陷在接受对氧磷暴露和mTBI组合的动物中最为明显，表明了损伤的累加效应。神经元存活率降低后，暴露于对氧磷或无mTBI损伤部位附近，而在齿状回门，细胞存活率降低，只有在小鼠暴露于对氧磷之前，持续mTBI。在暴露于mTBI和/或对氧磷的所有组中，齿状回的神经炎症增加。星形胶质细胞形态显着改变，在小鼠暴露于对氧磷之前，持续的mTBI。这些结果提供了进一步的支持假设OP暴露的影响，海湾战争后。这项研究揭示了OP暴露和mTBI的潜在叠加效应的额外见解，这可能导致现代战场上更严重的脑损伤。
Traumatic brain injury (TBI) is considered the most common neurological disorder among people under the age of 50. In modern combat zones, a combination of TBI and organophosphates (OP) can cause both fatal and long-term effects on the brain. We utilized a mouse closed-head TBI model induced by a weight drop device, along with OP exposure to paraoxon. Spatial and visual memory as well as neuron loss and reactive astrocytosis were measured 30 days after exposure to mild TBI (mTBI) and/or paraoxon. Molecular and cellular changes were assessed in the temporal cortex and hippocampus. Cognitive and behavioral deficits were most pronounced in animals that received a combination of paraoxon exposure and mTBI, suggesting an additive effect of the insults. Neuron survival was reduced in proximity to the injury site after exposure to paraoxon with or without mTBI, whereas in the dentate gyrus hilus, cell survival was only reduced in mice exposed to paraoxon prior to sustaining a mTBI. Neuroinflammation was increased in the dentate gyrus in all groups exposed to mTBI and/or to paraoxon. Astrocyte morphology was significantly changed in mice exposed to paraoxon prior to sustaining an mTBI. These results provide further support for assumptions concerning the effects of OP exposure following the Gulf War. This study reveals additional insights into the potentially additive effects of OP exposure and mTBI, which may result in more severe brain damage on the modern battlefield.
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影响因子：
4.6
作者：
Har-Even M;Rubovitch V;Ratliff WA;Richmond-Hacham B;Citron BA;Pick CG
通讯作者：
Pick CG
影响因子：
2.7
作者：
Dix, SL;Aggleton, JP
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Aggleton, JP
影响因子：
6.3
作者：
Delgado, E;McConnell, R;Wesseling, C
通讯作者：
Wesseling, C
影响因子：
3.1
作者：
Golderman, Valery;Shavit-Stein, Efrat;Maggio, Nicola
通讯作者：
Maggio, Nicola
影响因子：
3.6
作者：
Hogg, S
通讯作者：
Hogg, S