Post-Adipose-Derived Stem Cells (ADSC) Stimulated by Collagen Type V (Col V) Mitigate the Progression of Osteoarthritic Rabbit Articular Cartilage.
Post-Adipose-Derived Stem Cells (ADSC) Stimulated by Collagen Type V (Col V) Mitigate the Progression of Osteoarthritic Rabbit Articular Cartilage.
复制标题
DOI：
10.3389/fcell.2021.606890
复制
发表时间：
2021
影响因子：
5.5
通讯作者：
Teodoro WR
中科院分区：
文献类型：
--
作者：
Brindo da Cruz IC;Velosa APP;Carrasco S;Dos Santos Filho A;Tomaz de Miranda J;Pompeu E;Fernandes TL;Bueno DF;Fanelli C;Goldenstein-Schainberg C;Fabro AT;Fuller R;Silva PL;Capelozzi VL;Teodoro WR
胶原蛋白是软骨粘附和形成所必需的。在本研究中，组织学、免疫荧光、形态测定和qRT-PCR表明，V型胶原（Col V）刺激的脂肪源性干细胞（ADSCs）诱导手术性骨关节炎兔关节软骨（OA）退行性区II型胶原（Col II）显著增加。在体外研究了Col V对ADSC增殖分化的影响。adsc /Col V处理后，软骨相关基因Col2a1和Acan表达显著上调，Pou5fl表达下调。adsc /Col V处理后，通过苏木精和伊红（H&E）和红素O/Fast Green染色，兔的体内分析显示骨关节炎关节软骨再生的典型迹象。免疫组化染色显示，adsc /Col V处理后，细胞Col II纤维体积和Col II蛋白表达显著增加，凋亡Fas配体阳性显著降低。综上所述，Col II在兔手术性骨关节炎关节软骨中的表达较高；因此，ADSC/Col V可能是OA治疗的一个有希望的治疗靶点。
Collagen is essential for cartilage adhesion and formation. In the present study, histology, immunofluorescence, morphometry, and qRT-PCR suggested that adipose-derived stem cells (ADSCs) stimulated by type V collagen (Col V) induce a significant increase of type II collagen (Col II) in the degenerative area of surgical-induced osteoarthritic rabbit articular cartilage (OA). In vitro, the effects of Col V on the proliferation and differentiation of ADSC were investigated. The expression of the cartilage-related genes Col2a1 and Acan was significantly upregulated and Pou5fl was downregulated post-ADSC/Col V treatment. Post-ADSC/Col V treatment, in vivo analyses revealed that rabbits showed typical signs of osteoarthritic articular cartilage regeneration by hematoxylin and eosin (H&E) and Safranin O/Fast Green staining. Immunohistochemical staining demonstrated that the volume of Col II fibers and the expression of Col II protein were significantly increased, and apoptosis Fas ligand positive significantly decreased post-ADSC/Col V treatment. In conclusion, the expression of Col II was higher in rabbits with surgical-induced osteoarthritic articular cartilage; hence, ADSC/Col V may be a promising therapeutic target for OA treatment.
登录
查看更多内容
DOI：
10.1002/ar.23330
发表时间：
2016-05-01
影响因子：
2
作者：
Mak, Ki M.;Png, Chien Yi M.;Lee, Danielle J.
通讯作者：
Lee, Danielle J.
DOI：
10.1016/j.bbrc.2004.06.029
发表时间：
2004-07-30
影响因子：
3.1
作者：
Indrawattana, N;Chen, GP;Bunyaratvej, A
通讯作者：
Bunyaratvej, A
影响因子：
15.9
作者：
AIGNER, T;BERTLING, W;VONDERMARK, K
通讯作者：
VONDERMARK, K
DOI：
10.1016/j.biocel.2010.06.022
发表时间：
2010-10-01
影响因子：
4
作者：
Bertrand，Jessica;Cromme，Christoph;Pap，Thomas
通讯作者：
Pap，Thomas
DOI：
10.1002/jor.23571
发表时间：
2017-12
期刊：
Journal of orthopaedic research : official publication of the Orthopaedic Research Society
影响因子：
--
作者：
Johnston JM;Connizzo BK;Shetye SS;Robinson KA;Huegel J;Rodriguez AB;Sun M;Adams SM;Birk DE;Soslowsky LJ
通讯作者：
Soslowsky LJ