Relationship of HLA-DQA1 alleles and humoral antibody following measles vaccination.
Relationship of HLA-DQA1 alleles and humoral antibody following measles vaccination.
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麻疹疫苗接种后 HLA-DQA1 等位基因与体液抗体的关系。
DOI：
10.1016/s1201-9712(98)90116-3
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发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Lipsky,JJ
中科院分区：
文献类型：
--
作者：
Hayney,MS;Poland,GA;Jacobson,RM;Rabe,D;Schaid,DJ;Jacobsen,SJ;Lipsky,JJ
人类白细胞抗原(HLA)-DQA1位点与其他HLA II类位点相比仅具有中度多态性；然而，我们假设这些多态性可能在确定麻疹疫苗病毒的体液抗体反应中很重要。方法对881例15月龄前后接种MMR-II疫苗的学龄儿童进行麻疹抗体血清阳性率测定。所有受试者居住在没有麻疹病毒流行的地理区域。IgG抗体水平由麻疹特异性全病毒酶免疫分析法（EIA）测定（BioWhittaker, Walkersville， MD）。无应答者（IgG血清阴性或模棱两可）（n = 46）和高应答者（所有受试者IgG水平的前10百分位）（n = 64）使用序列特异性引物聚合酶链反应（PCR-SSP）进行HLA-DQA1分型。比较无应答者和高应答者之间的HLA-DQA1等位基因频率和纯合子率。结果无反应者和高反应者的等位基因频率总体分布差异有统计学意义（P = 0.05），无反应者的HLA-DQA1∗05等位基因过多（P = 0.017），高反应者的HLA-DQA1∗01等位基因过多（P = 0.013）。无应答者的纯合子率显著高于应答者（23.9% vs. 9.4%, P = 0.037）。结论hla - dqa1等位基因与麻疹疫苗抗体应答有重要关系。具体来说，HLA-DQA1∗05等位基因的携带与无应答相关，HLA-DQA1∗01等位基因的携带与高应答相关。此外，HLA-DQA1纯合性与麻疹疫苗抗体反应差显著相关。
BackgroundThe human leukocyte antigen (HLA)-DQA1 locus is only moderately polymorphic compared to other HLA class II loci; however, we hypothesized that these polymorphisms could be important in determining the humoral antibody response to measles vaccine virus.MethodsThe seroprevalence of measles antibody was determined in 881 school children who had been immunized with MMR-II at age approximately 15 months. All subjects resided in a geographic area with no circulating measles virus. The IgG antibody levels were determined by a measles-specific whole virus enzyme immunoassay (EIA) (BioWhittaker, Walkersville, MD). Subjects who were nonresponders (IgG seronegative or equivocal) (n = 46) and hyperresponders (upper 10th percentile of IgG levels of all subjects) (n = 64) were HLA-DQA1 typed using polymerase chain reaction with sequence-specific primers (PCR-SSP). The HLA-DQA1 allele frequencies, as well as homozygosity rates, were compared between the nonresponders and hyperresponders.ResultsThe overall allele frequency distribution of alleles between the nonresponders and hyperresponders was significantly different (P = 0.05), with nonresponders having an excess of HLA-DQA∗05 alleles (P = 0.017) and hyperresponders having an excess of HLA-DQA1∗01 alleles (P = 0.013). The homozygosity rate among nonresponders was significantly higher than among hyperresponders (23.9% vs. 9.4%, P = 0.037).ConclusionHLA-DQA1 alleles have important associations with the antibody response to measles vaccine. Specifically, the carriage of the HLA-DQA1∗05 alleles is associated with nonresponse and that of HLA-DQA1∗01 alleles with hyperresponse. In addition, HLA-DQA1 homozygosity is significantly associated with poor antibody response to measles vaccine.
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影响因子：
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作者：
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作者：
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DOI：
--
发表时间：
1997
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
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作者：
Calvo-Calle,JM;Hammer,J;Sinigaglia,F;Clavijo,P;Moya-Castro,ZR;Nardin,EH
通讯作者：
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