USP22 is useful as a novel molecular marker for predicting disease progression and patient prognosis of oral squamous cell carcinoma.
USP22 is useful as a novel molecular marker for predicting disease progression and patient prognosis of oral squamous cell carcinoma.
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USP22 可作为预测口腔鳞状细胞癌疾病进展和患者预后的新型分子标记物
DOI：
10.1371/journal.pone.0042540
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Zhang B
中科院分区：
文献类型：
--
作者：
Piao S;Liu Y;Hu J;Guo F;Ma J;Sun Y;Zhang B
泛素特异性蛋白酶22（USP 22）作为一种潜在的标志物在肿瘤学领域的重要性日益增加。本研究旨在探讨USP 22在口腔鳞状细胞癌（OSCC）中的作用及其与潜在靶点的关系。采用免疫组化法检测319例口腔鳞癌组织中USP 22蛋白的表达，并与42例健康对照组进行比较。通过分析异常表达蛋白的临床相关性和预后意义，寻找新的口腔鳞癌生物标志物。319例口腔鳞癌组织中USP 22阳性表达率为63.32%。从非癌粘膜到原发癌、从癌组织到淋巴结转移灶，USP 22蛋白表达水平均呈上调趋势（P<0.001）。统计学分析显示USP 22阳性表达与淋巴结转移、Ki 67、考克斯-2及复发呈正相关。此外，对于淋巴结阳性的患者，与USP 22阴性表达的患者相比，USP 22阳性表达的患者具有显著较差的结果。多因素考克斯回归分析显示USP 22表达水平是影响总生存期和无病生存期的独立预后因素（分别为P<0.001和P<0.001）。通过MTT和软琼脂测定法评价，USP 22表达降低的癌细胞表现出增殖和集落形成降低。据我们所知，这是第一个确定USP 22表达与OSCC预后关系的研究。我们发现USP 22的高表达与口腔鳞癌的不良预后有关。USP 22可能是一种新的和有用的预后标志物的口腔鳞癌。
The significance of ubiquitin-specific protease 22 (USP22) as a potential marker has been growing in the field of oncology. The aim of this study was to investigate the role of USP22 and the association with its potential targets in oral squamous cell carcinoma (OSCC). Immunohistochemistry was used to determine the expression of USP22 protein in 319 OSCC patients in comparison with 42 healthy controls. The clinical correlations and prognostic significance of the aberrantly expressed protein was evaluated to identify novel biomarker of OSCC. The incidence of positive USP22 expression was 63.32% in 319 conventional OSCC tissues. The protein expression level of USP22 was concomitantly up-regulated from non-cancerous mucosa to primary carcinoma and from carcinomas to lymph node metastasis (P<0.001). Moreover, statistical analysis showed that positive USP22 expression was positively related to lymph node metastasis, Ki67, Cox-2 and recurrence. Furthermore, it was shown that patients with positive USP22 expression had significantly poorer outcome compared with patients with negative expression of USP22 for patients with positive lymph nodes. Multivariate Cox regression analysis revealed that USP22 expression level was an independent prognostic factor for both overall survival and disease-free survival (P<0.001 and P<0.001, respectively). Cancer cells with reduced USP22 expression exhibited reduced proliferation and colony formation evaluated by MTT and soft agar assays. To our knowledge, this is the first study that determines the relationship between USP22 expression and prognosis in OSCC. We found that increased expression of USP22 is associated with poor prognosis in OSCC. USP22 may represent a novel and useful prognostic marker for OSCC.
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影响因子：
4.3
作者：
Zhang, Xiao-Yong;Pfeiffer, Harla K.;McMahon, Steven B.
通讯作者：
McMahon, Steven B.
影响因子：
2.8
作者：
Xu, Hui;Liu, Yan-Long;Dong, Xin-Shu
通讯作者：
Dong, Xin-Shu
影响因子：
2.5
作者：
Liu, Yan-Long;Yang, Yan-Mei;Dong, Xin-Shu
通讯作者：
Dong, Xin-Shu
影响因子：
3.1
作者：
Wolff, D;Hassfeld, S;Hofele, C
通讯作者：
Hofele, C
影响因子：
3.7
作者：
Lv T;Yuan D;Miao X;Lv Y;Zhan P;Shen X;Song Y
通讯作者：
Song Y