Cerebral endothelial derived vascular endothelial growth factor promotes the migration but not the proliferation of oligodendrocyte precursor cells in vitro.
Cerebral endothelial derived vascular endothelial growth factor promotes the migration but not the proliferation of oligodendrocyte precursor cells in vitro.
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DOI：
10.1016/j.neulet.2012.02.004
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发表时间：
2012-03-28
影响因子：
2.5
通讯作者：
Arai K
中科院分区：
文献类型：
--
作者：
Hayakawa K;Seo JH;Pham LD;Miyamoto N;Som AT;Guo S;Kim KW;Lo EH;Arai K
在灰质中，已知脑内皮为邻近细胞如神经元提供营养支持。然而，从脑内皮到白色细胞的信号传导仍有待阐明。在这里，我们表明，血管内皮细胞分泌的血管内皮生长因子（VEGF-A）促进迁移，但不是少突胶质细胞前体细胞（OPCs）的增殖。从新生大鼠皮层获得培养的OPC，并且用脑内皮细胞的条件培养基处理增加OPC增殖和迁移。重要的是，与Flk-1（VEGF-受体2）的抗中和抗体共同治疗抑制OPC运动，但不影响OPC繁殖。Western blot和流式细胞仪分析证实，我们培养的脑内皮细胞产生VEGF-A和我们培养的OPCs表达Flk-1。总之，我们目前的数据表明，脑内皮细胞是支持少突胶质细胞谱系细胞和VEGF-A可能参与内皮细胞-OPC细胞-细胞信号转导。这种现象可能是重要的白色物质的稳态。
In gray matter, cerebral endothelium is known to provide trophic support for neighboring cells such as neurons. However, signaling from cerebral endothelium to white matter cells remains to be elucidated. Here, we show that vascular endothelial growth factor (VEGF-A) secreted from cerebral endothelial cells promotes the migration but not the proliferation of oligodendrocyte precursor cells (OPCs). Cultured OPCs were obtained from newborn rat cortex, and treatment with conditioned culture media of cerebral endothelial cells increased the OPC proliferation and migration. Importantly, co-treatment with anti-neutralizing antibody for Flk-1 (VEGF-receptor2) inhibited OPC movement but did not affect OPC propagation. Western blot and flow cytometry analyses confirmed that our cultured cerebral endothelial cells produced VEGF-A and our cultured OPCs expressed Flk-1. Taken together, our current data suggest that cerebral endothelium is supportive for oligodendrocyte lineage cells and VEGF-A may participate in the endothelium-OPC cell-cell signaling. This phenomenon may be important for white matter homeostasis.
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DOI：
10.1523/jneurosci.5086-09.2010
发表时间：
2010-05-05
期刊：
The Journal of neuroscience : the official journal of the Society for Neuroscience
影响因子：
--
作者：
Paez PM;Fulton DJ;Spreur V;Handley V;Campagnoni AT
通讯作者：
Campagnoni AT
影响因子：
5.3
作者：
Ohab, John J.;Fleming, Sheila;Carmichael, S. Thomas
通讯作者：
Carmichael, S. Thomas
影响因子：
5.3
作者：
Arai, Ken;Lo, Eng H.
通讯作者：
Lo, Eng H.
影响因子：
2.9
作者：
Ohya, Wakana;Funakoshi, Hiroshi;Nakamura, Toshikazu
通讯作者：
Nakamura, Toshikazu
影响因子：
8.3
作者：
Krupinski, J;Kumar, P;Kaluza, J
通讯作者：
Kaluza, J