Down-regulation of inhibitor of apoptosis levels provides competence for steroid-triggered cell death.
Down-regulation of inhibitor of apoptosis levels provides competence for steroid-triggered cell death.
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DOI：
10.1083/jcb.200703206
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发表时间：
2007-07-02
影响因子：
7.8
通讯作者：
Bashirullah, Arash
中科院分区：
文献类型：
--
作者：
Yin, Viravuth P.;Thummel, Carl S.;Bashirullah, Arash
在果蝇变态过程中，类固醇激素蜕皮激素的脉冲触发幼虫唾液腺的破坏，通过转录级联在诱导收割者(RPR)和头部退化缺陷(HID)时汇聚，导致caspase激活和细胞死亡。我们发现CREB结合蛋白(CBP)转录辅助因子是唾液腺细胞死亡所必需的。我们发现，CBP在变态开始前1天对中三龄蜕皮激素脉冲做出明显反应，而CBP是下调果蝇凋亡抑制因子1(DIAP1)的必要和充分条件。只有在DIAP1水平降低后，唾液腺才能通过RPR/HID介导的细胞死亡而死亡。在此之前，高水平的DIAP1阻止唾液腺细胞死亡，即使在RPR异位表达的情况下也是如此。这项研究表明，自然发生的凋亡抑制水平的变化对于调节发育过程中的细胞死亡至关重要。它还为获得类固醇信号通路中的活性提供了分子机制。
A pulse of the steroid hormone ecdysone triggers the destruction of larval salivary glands during Drosophila metamorphosis through a transcriptional cascade that converges on reaper (rpr) and head involution defective (hid) induction, resulting in caspase activation and cell death. We identify the CREB binding protein (CBP) transcriptional cofactor as essential for salivary gland cell death. We show that CBP acts 1 d before the onset of metamorphosis in apparent response to a mid-third instar ecdysone pulse, when CBP is necessary and sufficient for down-regulation of the Drosophila inhibitor of apoptosis 1 (DIAP1). It is only after DIAP1 levels are reduced that salivary glands become competent to die through rpr/hid-mediated cell death. Before this time, high levels of DIAP1 block salivary gland cell death, even in the presence of ectopic rpr expression. This study shows that naturally occurring changes in inhibitor of apoptosis levels can be critical for regulating cell death during development. It also provides a molecular mechanism for the acquisition of competence in steroid signaling pathways.
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