Lineage-tracing and translatomic analysis of damage-inducible mitotic cochlear progenitors identifies candidate genes regulating regeneration.
Lineage-tracing and translatomic analysis of damage-inducible mitotic cochlear progenitors identifies candidate genes regulating regeneration.
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DOI：
10.1371/journal.pbio.3001445
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发表时间：
2021-11
期刊：
影响因子：
9.8
通讯作者：
Cheng AG
中科院分区：
文献类型：
--
作者：
Udagawa T;Atkinson PJ;Milon B;Abitbol JM;Song Y;Sperber M;Huarcaya Najarro E;Scheibinger M;Elkon R;Hertzano R;Cheng AG
Cochlear supporting cells (SCs) are glia-like cells critical for hearing function. In the neonatal cochlea, the greater epithelial ridge (GER) is a mitotically quiescent and transient organ, which has been shown to nonmitotically regenerate SCs. Here, we ablated Lgr5+ SCs using Lgr5-DTR mice and found mitotic regeneration of SCs by GER cells in vivo. With lineage tracing, we show that the GER houses progenitor cells that robustly divide and migrate into the organ of Corti to replenish ablated SCs. Regenerated SCs display coordinated calcium transients, markers of the SC subtype inner phalangeal cells, and survive in the mature cochlea. Via RiboTag, RNA-sequencing, and gene clustering algorithms, we reveal 11 distinct gene clusters comprising markers of the quiescent and damaged GER, and damage-responsive genes driving cell migration and mitotic regeneration. Together, our study characterizes GER cells as mitotic progenitors with regenerative potential and unveils their quiescent and damaged translatomes. Cochlear supporting cells are glia-like cells essential for hearing. Genetic ablation of Lgr5+ supporting cells reveals a population of damage inducible, mitotically activated progenitors in the greater epithelial ridge, which can divide and migrate into the organ of Corti to replenish ablated supporting cells. Translatomic analyses provide insights into the genes that may regulate this regenerative potential.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
1.5
作者：
Doerflinger, NH;Macklin, WB;Popko, B
通讯作者：
Popko, B
影响因子：
2.4
作者：
Basch ML;Brown RM 2nd;Jen HI;Groves AK
通讯作者：
Groves AK
DOI：
10.1002/lary.28765
发表时间：
2021-09
期刊：
The Laryngoscope
影响因子：
--
作者：
Hertzano R;Gwilliam K;Rose K;Milon B;Matern MS
通讯作者：
Matern MS
影响因子：
7.7
作者：
Basch ML;Brown RM 2nd;Jen HI;Semerci F;Depreux F;Edlund RK;Zhang H;Norton CR;Gridley T;Cole SE;Doetzlhofer A;Maletic-Savatic M;Segil N;Groves AK
通讯作者：
Groves AK