Emergence of a new cholera pandemic: molecular analysis of virulence determinants in Vibrio cholerae O139 and development of a live vaccine prototype.-猫眼课题宝

新霍乱大流行的出现：霍乱弧菌 O139 毒力决定因素的分子分析和活疫苗原型的开发。

DOI：

10.1093/infdis/170.2.278

发表时间：

1994

期刊：

The Journal of infectious diseases

影响因子：

通讯作者：

中科院分区：

文献类型：

作者：

Waldor,MK;Mekalanos,JJ

1992 年 10 月，一种非 Ol 霍乱弧菌菌株出现，成为印度和孟加拉国流行霍乱的原因。这种抗原性新颖的克隆被命名为血清群 0139，同义词孟加拉。自出现以来，V. cholerae0139 已在印度次大陆及其以外地区造成大规模霍乱疫情。对临床分离株毒力决定因素的分子分析表明，0139 菌株与 El Tor 01 菌株高度相关。与其他非 O 1 菌株不同，0139 菌株携带多个拷贝的霍乱毒素遗传元件以及毒素共调节菌毛的基因。这些结果指导了 liveV 的构建。霍乱0139疫苗原型通过删除至少四个特定毒力决定簇（ctxA、ace、zot和cep）以及参与位点特异性和同源重组的其他因子（RS1、attRS1和recA）的基因。希望这种减毒活疫苗有助于控制霍乱霍乱的大流行传播。霍乱0139。

In October 1992, a non-Ol strain ofVibrio choleraeemerged as a cause of epidemic cholera in India and Bangladesh. This antigenically novel clone has been designated serogroup 0139 synonym Bengal. Since its emergence,V. cholerae0139 has caused a massive cholera epidemic throughout and beyond the Indian subcontinent. Molecular analysis of virulence determinants in clinical isolates suggests that 0139 strains are highly related to El Tor 01 strains. Unlike other non-O 1 strains, 0139 strains carry multiple copies of the cholera toxin genetic element and also genes for the toxin-coregulated pilus. These results guided construction of a liveV. cholerae0139 vaccine prototype through deletion of genes for at least four specific virulence determinants(ctxA, ace, zot,andcep)as well as other factors involved in site-specific and homologous recombination (RS1, attRS1, andrecA).It is hoped that this attenuated live vaccine will help control the pandemic spread ofV. cholerae 0139.