Rapid measurement of ageing by automated monitoring of movement of C. elegans populations.
Rapid measurement of ageing by automated monitoring of movement of C. elegans populations.
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DOI：
10.1007/s11357-023-00998-w
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发表时间：
2024-04
期刊：
影响因子：
5.6
通讯作者：
Weinkove, David
中科院分区：
文献类型：
--
作者：
Zavagno, Giulia;Raimundo, Adelaide;Kirby, Andy;Saunter, Christopher;Weinkove, David
寻找减缓衰老和保持人类健康的新干预措施是我们这个时代的一个巨大挑战。秀丽隐杆线虫提供了一种快速的体内方法来确定化合物是否延长其2至3周的寿命。测量寿命是监测衰老的标准方法，但延长寿命的化合物不一定能保持健康。在这里，我们描述了C的自动监控。elegans运动从早期到中期成年作为一个更快的健康为基础的方法来衡量老化。使用WormGazer ™技术，多个培养皿，每个培养皿含有几个C.通过一组易于缩放的照相机同时且非侵入性地对秀丽线虫蠕虫进行成像。这种方法表明，大多数功能下降的C。在成年期的第一周内发生。我们发现7天的成像足以测量磺胺甲恶唑延缓衰老的剂量依赖性功效，而平行寿命实验需要40天。了解延缓衰老的干预措施的任何负面后果是重要的。我们发现，长寿的突变年龄-1（hx546）保持活跃的时间比野生型更长，但它在成年早期移动较慢。因此，对运动的持续分析可以迅速确定减缓衰老的干预措施，同时揭示对健康的任何负面影响。在线版本包含补充材料，可通过10.1007/s11357 - 023 - 00998-w获得。
Finding new interventions that slow ageing and maintain human health is a huge challenge of our time. The nematode Caenorhabditis elegans offers a rapid in vivo method to determine whether a compound extends its 2 to 3-week lifespan. Measuring lifespan is the standard method to monitor ageing, but a compound that extends lifespan will not necessarily maintain health. Here, we describe the automated monitoring of C. elegans movement from early to mid-adulthood as a faster healthspan-based method to measure ageing. Using the WormGazer™ technology, multiple Petri dishes each containing several C. elegans worms are imaged simultaneously and non-invasively by an array of cameras that can be scaled easily. This approach demonstrates that most functional decline in C. elegans occurs during the first week of adulthood. We find 7 days of imaging is sufficient to measure the dose-dependent efficacy of sulfamethoxazole to slow ageing, compared to 40 days required for a parallel lifespan experiment. Understanding any negative consequences of interventions that slow ageing is important. We show that the long-lived mutant age-1(hx546) stays active for longer than the wild type but it moves slower in early adulthood. Thus, continuous analysis of movement can rapidly identify interventions that slow ageing while simultaneously revealing any negative effects on health. The online version contains supplementary material available at 10.1007/s11357-023-00998-w.
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影响因子：
64.5
作者：
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通讯作者：
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影响因子：
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作者：
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