Porcine Vocal Fold Lamina Propria-Derived Biomaterials Modulate TGF-β1-Mediated Fibroblast Activation in Vitro.
Porcine Vocal Fold Lamina Propria-Derived Biomaterials Modulate TGF-β1-Mediated Fibroblast Activation in Vitro.
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DOI：
10.1021/acsbiomaterials.9b01837
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发表时间：
2020-03-09
影响因子：
5.8
通讯作者：
Freytes DO
中科院分区：
文献类型：
--
作者：
Mora-Navarro C;Badileanu A;Gracioso Martins AM;Ozpinar EW;Gaffney L;Huntress I;Harrell E;Enders JR;Peng X;Branski RC;Freytes DO
声带固有层（VFLP）是声带（VF）的最外层之一，由组织特异性细胞外基质（ECM）蛋白组成，并且非常容易受到损伤。各种生物材料已经在临床上测试以治疗声音障碍（例如，水凝胶、脂肪、透明质酸），但VF功能的令人满意的恢复仍然难以捉摸。VF中的纤维化或瘢痕形成是一个主要挑战，并且需要开发和改进促进VF的愈合和正常功能的新疗法。先前已报道来源于天然组织的可注射水凝胶具有优于合成水凝胶的主要优点，包括建设性组织重塑和减少的瘢痕组织形成。本研究旨在表征脱细胞猪VFLP-ECM支架的组成以及源自VFLP-ECM水凝胶的水凝胶的细胞相容性和潜在的抗纤维化特性。此外，我们从VFLP-ECM中分离出潜在的基质结合囊泡（MBV）和大分子，它们也在TGF-β1刺激下下调平滑肌肌动蛋白ACTA 2。结果提供了VFLP-ECM的独特蛋白质组成以及VFLP-ECM组分与转化为注射形式时体外观察到的转化生长因子-β 1（TGF-β1）信号传导抑制之间的潜在联系的证据。
The vocal fold lamina propria (VFLP), one of the outermost layers of the vocal fold (VF), is composed of tissue-specific extracellular matrix (ECM) proteins and is highly susceptible to injury. Various biomaterials have been clinically tested to treat voice disorders (e.g., hydrogels, fat, hyaluronic acid), but satisfactory recovery of the VF functionality remains elusive. Fibrosis or scar formation in the VF is a major challenge, and the development and refinement of novel therapeutics that promote the healing and normal function of the VF are needed. Injectable hydrogels derived from native tissues have been previously reported with major advantages over synthetic hydrogels, including constructive tissue remodeling and reduced scar tissue formation. This study aims to characterize the composition of a decellularized porcine VFLP-ECM scaffold and the cytocompatibility and potential anti-fibrotic properties of a hydrogel derived from VFLP-ECM hydrogel. In addition, we isolated potential matrix-bound vesicles (MBVs) and macromolecules from the VFLP-ECM that also downregulated smooth muscle actin ACTA2 under TGF-β1 stimulation. The results provide evidence of the unique protein composition of VFLP-ECM and the potential link between the components of VFLP-ECM and the inhibition of transforming growth factor-beta 1 (TGF-β1) signaling observed in vitro when transformed into injectable forms.
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DOI：
10.1002/lary.25673
发表时间：
2016-05
期刊：
The Laryngoscope
影响因子：
--
作者：
Branski RC;Bing R;Kraja I;Amin MR
通讯作者：
Amin MR
影响因子：
6.5
作者：
Achterberg, Volker F.;Buscemi, Lara;Hinz, Boris
通讯作者：
Hinz, Boris
影响因子：
4
作者：
Bartlett, Rebecca S.;Thibeault, Susan L.;Prestwich, Glenn D.
通讯作者：
Prestwich, Glenn D.
DOI：
10.1111/j.2517-6161.1995.tb02031.x
发表时间：
1995-01-01
影响因子：
5.8
作者：
BENJAMINI, Y;HOCHBERG, Y
通讯作者：
HOCHBERG, Y
影响因子：
14
作者：
Freytes, Donald O.;Martin, Jeffrey;Badylak, Stephen F.
通讯作者：
Badylak, Stephen F.