Regulatory Role of Zinc in Allergic Rhinitis through the IL-33/ST2 Pathway.
Regulatory Role of Zinc in Allergic Rhinitis through the IL-33/ST2 Pathway.
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锌通过 IL-33/ST2 途径对过敏性鼻炎的调节作用
DOI：
10.1155/2022/3718317
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发表时间：
2022
影响因子：
--
通讯作者：
Ma R
中科院分区：
文献类型：
--
作者：
Wen W;Yang F;Shen X;Feng N;Ha H;Ma R
本研究旨在探讨血清锌和细胞因子白细胞介素13（IL-13）、IL-33在变应性鼻炎（AR）患者中的表达，并观察锌对青蒿变应原刺激的肥大细胞P815细胞因子及其通路蛋白的影响。IL-33/肿瘤发生2（ST 2）通路的抑制。本研究旨在探讨锌在AR中可能的调节作用，为确定AR的病因和治疗提供新的思路。 选择2018年3月至9月接受治疗的AR患者作为研究对象，同期选择50名健康人作为对照组。收集所有患者的血清样本，并对AR患者的血清样本进行过敏原检测。采用酶联免疫吸附法检测IL-13和IL-33的表达，电感耦合等离子体质谱法检测血清锌水平。采用CCK-8细胞计数试剂盒检测P815肥大细胞增殖情况;采用western blot检测ST 2、p38、p65蛋白的表达。 共有92名AR患者纳入研究;其中52名为轻度AR，40名为中度AR。AR患者的主要变应原为蒿属，阳性率为53.26%。AR患者血清锌离子水平降低，IL-13、IL-33表达增加。Art.处理P815肥大细胞后，细胞上清液中IL-33的表达呈浓度依赖性增加，受体ST 2的表达增加，下游p38和p65蛋白的表达增加。ZnSO 4处理后，细胞上清中IL-33的表达下降，ST 2、p38和p65蛋白的表达下降。 AR患者血清锌水平降低。在IL-33/ST 2信号通路中，硫酸锌可降低Art诱导的肥大细胞超敏反应。
We aimed to investigate the expression of serum zinc and cytokines interleukin- (IL-) 13 and IL-33 in patients with allergic rhinitis (AR) and observe the effects of zinc on cytokines and pathway proteins in P815 mast cells stimulated by Artemisia annua allergen (Art.) in the IL-33/suppression of the tumorigenicity 2 (ST2) pathway. We also aimed to explore the possible regulatory role of zinc in AR and provide new ideas to determine the etiology and treatment of AR. AR patients treated from March to September in 2018 were selected as the research participants, and 50 healthy people in the same period were selected as the control group. Serum samples of all patients were collected, and those of AR patients were tested for the presence of allergens. The expression of IL-13 and IL-33 was detected by performing an enzyme-linked immunosorbent assay, while the serum zinc level was detected by conducting an inductively coupled plasma mass spectrometry. The cell counting kit (CCK-8) was used to detect the proliferation of P815 mast cells, and western blot was used to detect the expression of ST2, p38, and p65 proteins. A total of 92 AR patients were included in the study; of them, 52 had mild AR, while 40 had moderate AR. The primary allergen found in AR patients was Artemisia, and the positivity rate was 53.26%. The serum zinc ion level of AR patients decreased, and the expression of IL-13 and IL-33 increased. After Art. was used to treat P815 mast cells, the expression of IL-33 in the cell supernatant increased in a concentration-dependent manner, the expression of receptor ST2 increased, and the expression of downstream p38 and p65 proteins increased. However, after treatment with ZnSO4, the expression of IL-33 in the cell supernatant decreased, and the expression of ST2, p38, and p65 protein decreased. The serum zinc level of AR patients decreased. In the IL-33/ST2 pathway, ZnSO4 can reduce the hypersensitivity of mast cells induced by Art.
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影响因子：
4.3
作者：
Endo, Shuichiro;Hochman, Daniel J.;Midoro-Horiuti, Terumi;Goldblum, Randall M.;Brooks, Edward G.
通讯作者：
Brooks, Edward G.
影响因子：
13.6
作者：
Wang, Long;Yang, Tao;Yu, Feng
通讯作者：
Yu, Feng
影响因子：
1.3
作者：
Gluck, Joanna;Rymarczyk, Barbara;Rogala, Barbara
通讯作者：
Rogala, Barbara
影响因子：
12.4
作者：
Nurmatov, U.;Nwaru, B. I.;Sheikh, A.
通讯作者：
Sheikh, A.
影响因子：
6.1
作者：
Sakashita, M.;Yoshimoto, T.;Tamari, M.
通讯作者：
Tamari, M.