Comparative transcriptomics reveals desynchronisation of gene expression during the floral transition between Arabidopsis and Brassica rapa cultivars.
Comparative transcriptomics reveals desynchronisation of gene expression during the floral transition between Arabidopsis and Brassica rapa cultivars.
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DOI：
10.1017/qpb.2021.6
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发表时间：
2021
影响因子：
--
通讯作者：
Morris, Richard J.
中科院分区：
文献类型：
--
作者：
Calderwood, Alexander;Hepworth, Jo;Woodhouse, Shannon;Bilham, Lorelei;Jones, D. Marc;Tudor, Eleri;Ali, Mubarak;Dean, Caroline;Wells, Rachel;Irwin, Judith A.;Morris, Richard J.
比较转录学可以用来将对基因调控网络的理解从模型系统转换到较少研究的物种。在这里，我们使用RNA-Seq来确定和比较模式物种拟南芥和近缘作物油菜在花期转换过程中的基因表达动态。我们发现不同的基因需要不同的曲线配准函数，这表明拟南芥和油菜之间不存在单一的共同的“发育时间”。通过对拟南芥和油菜之间以及两个油菜材料之间的详细比较，揭示了关键的花整合基因SOC1的不同调控模式，并且油菜材料中的花转换是由不同的途径触发的。我们的研究加深了对快速循环甘蓝型油菜开花时间调控网络的机械性理解，并强调了注册方法对于比较发育基因表达数据的重要性。
Comparative transcriptomics can be used to translate an understanding of gene regulatory networks from model systems to less studied species. Here, we use RNA-Seq to determine and compare gene expression dynamics through the floral transition in the model species Arabidopsis thaliana and the closely related crop Brassica rapa. We find that different curve registration functions are required for different genes, indicating that there is no single common ‘developmental time’ between Arabidopsis and B. rapa. A detailed comparison between Arabidopsis and B. rapa and between two B. rapa accessions reveals different modes of regulation of the key floral integrator SOC1, and that the floral transition in the B. rapa accessions is triggered by different pathways. Our study adds to the mechanistic understanding of the regulatory network of flowering time in rapid cycling B. rapa and highlights the importance of registration methods for the comparison of developmental gene expression data.
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影响因子：
64.8
作者：
Fu, XD;Harberd, NP
通讯作者：
Harberd, NP
影响因子：
4.5
作者：
He J;Xu M;Willmann MR;McCormick K;Hu T;Yang L;Starker CG;Voytas DF;Meyers BC;Poethig RS
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Poethig RS
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9.2
作者：
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通讯作者：
Wigge, Philip A.
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del Olmo, Ivan;Poza-Viejo, Laura;Crevillen, Pedro
通讯作者：
Crevillen, Pedro
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2.7
作者：
Franks, Steven J.;Perez-Sweeney, Beatriz;Litt, Amy
通讯作者：
Litt, Amy