The yeast GRD20 gene is required for protein sorting in the trans-Golgi network/endosomal system and for polarization of the actin cytoskeleton.
The yeast GRD20 gene is required for protein sorting in the trans-Golgi network/endosomal system and for polarization of the actin cytoskeleton.
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酵母 GRD20 基因是跨高尔基体网络/内体系统中的蛋白质分选和肌动蛋白细胞骨架极化所必需的。
DOI：
10.1091/mbc.10.12.4263
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发表时间：
1999
影响因子：
3.3
通讯作者：
Nothwehr,SF
中科院分区：
文献类型：
--
作者：
Spelbrink,RG;Nothwehr,SF
The proper localization of resident membrane proteins to thetrans-Golgi network (TGN) involves mechanisms for both TGN retention and retrieval from post-TGN compartments. In this study we report identification of a new gene,GRD20, involved in protein sorting in the TGN/endosomal system ofSaccharomyces cerevisiae. A strain carrying a transposon insertion allele ofGRD20exhibited rapid vacuolar degradation of the resident TGN endoprotease Kex2p and aberrantly secreted ∼50% of the soluble vacuolar hydrolase carboxypeptidase Y. The Kex2p mislocalization and carboxypeptidase Y missorting phenotypes were exhibited rapidly after loss of Grd20p function ingrd20temperature-sensitive mutant strains, indicating that Grd20p plays a direct role in these processes. Surprisingly, little if any vacuolar degradation was observed for the TGN membrane proteins A-ALP and Vps10p, underscoring a difference in trafficking patterns for these proteins compared with that of Kex2p. Agrd20null mutant strain exhibited extremely slow growth and a defect in polarization of the actin cytoskeleton, and these two phenotypes were invariably linked in a collection of randomly mutagenizedgrd20alleles.GRD20encodes a hydrophilic protein that partially associates with the TGN. The discovery ofGRD20suggests a link between the cytoskeleton and function of the yeast TGN.
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DOI：
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发表时间：
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期刊：
Science (New York, N.Y.)
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DOI：
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发表时间：
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期刊：
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影响因子：
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通讯作者：
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