Acute Injection of Omega-3 Triglyceride Emulsion Provides Very Similar Protection as Hypothermia in a Neonatal Mouse Model of Hypoxic-Ischemic Brain Injury.
Acute Injection of Omega-3 Triglyceride Emulsion Provides Very Similar Protection as Hypothermia in a Neonatal Mouse Model of Hypoxic-Ischemic Brain Injury.
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DOI：
10.3389/fneur.2020.618419
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发表时间：
2020
影响因子：
3.4
通讯作者：
Deckelbaum RJ
中科院分区：
文献类型：
--
作者：
Manual Kollareth DJ;Zirpoli H;Ten VS;Deckelbaum RJ
治疗性低温（HT）是目前公认的新生儿窒息的治疗方法，也是治疗成人卒中的一种有前途的策略。我们以前报道过，急性给药二十二碳六烯酸（DHA）甘油三酯乳剂（tri-DHA）保护新生小鼠免受缺氧缺血（HI）损伤。我们质疑HT和tri-DHA联合治疗是否会在保护大脑免受HI损伤方面产生协同作用。将经受HI损伤的新生小鼠（10日龄）置于温度控制室中4小时的HT（直肠温度31-32°C）或常温（NT，直肠温度37°C）。在HT开始之前和之后1小时，用tri-DHA（0.375 g tri-DHA/kg bw，两次注射）处理小鼠。我们观察到，在HI损伤后立即开始的HT减少了脑梗死体积，与tri-DHA治疗相似（约50%）。此外，HI损伤后延迟2小时的HT提供神经保护（%梗塞体积：31.4 ± 4.1对18.8 ± 4.6 HT），而延迟4小时的HT不能保护免受HI损伤（%梗塞体积：30.7 ± 5.0对31.3 ± 5.6 HT）。与单独HT相比，在HI损伤后0或2小时开始的HT加tri-DHA组合治疗没有进一步减少梗死体积。我们的研究结果表明，HT提供了类似程度的神经保护HI损伤相比，三DHA治疗。HT只能在三级护理中心提供，需要密切监测，并可能产生不良影响。相比之下，三-DHA治疗可能有利于为HI损伤后的患者提供可行且有效的策略。
Therapeutic hypothermia (HT) is a currently accepted treatment for neonatal asphyxia and is a promising strategy in adult stroke therapy. We previously reported that acute administration of docosahexaenoic acid (DHA) triglyceride emulsion (tri-DHA) protects against hypoxic-ischemic (HI) injury in neonatal mice. We questioned if co-treatment with HT and tri-DHA would achieve synergic effects in protecting the brain from HI injury. Neonatal mice (10-day old) subjected to HI injury were placed in temperature-controlled chambers for 4 h of either HT (rectal temperature 31–32°C) or normothermia (NT, rectal temperature 37°C). Mice were treated with tri-DHA (0.375 g tri-DHA/kg bw, two injections) before and 1 h after initiation of HT. We observed that HT, beginning immediately after HI injury, reduced brain infarct volume similarly to tri-DHA treatment (~50%). Further, HT delayed 2 h post-HI injury provided neuroprotection (% infarct volume: 31.4 ± 4.1 vs. 18.8 ± 4.6 HT), while 4 h delayed HT did not protect against HI insult (% infarct volume: 30.7 ± 5.0 vs. 31.3 ± 5.6 HT). HT plus tri-DHA combination treatment beginning at 0 or 2 h after HI injury did not further reduce infarct volumes compared to HT alone. Our results indicate that HT offers similar degrees of neuroprotection against HI injury compared to tri-DHA treatment. HT can only be provided in tertiary care centers, requires intense monitoring and can have adverse effects. In contrast, tri-DHA treatment may be advantageous in providing a feasible and effective strategy in patients after HI injury.
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影响因子：
2.7
作者：
Manual Kollareth DJ;Chang CL;Hansen IH;Deckelbaum RJ
通讯作者：
Deckelbaum RJ
影响因子：
3
作者：
Cilio, Maria Roberta;Ferriero, Donna M.
通讯作者：
Ferriero, Donna M.
DOI：
10.1007/s11936-010-0079-4
发表时间：
2010-08-01
影响因子：
--
作者：
Defilippis, Andrew Paul;Blaha, Michael J;Jacobson, Terry A
通讯作者：
Jacobson, Terry A
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影响因子：
4.6
作者：
An H;Duan Y;Wu D;Yip J;Elmadhoun O;Wright JC;Shi W;Liu K;He X;Shi J;Jiang F;Ji X;Ding Y
通讯作者：
Ding Y
影响因子：
5.1
作者：
Akula, Vishnu Priya;Joe, Priscilla;Van Meurs, Krisa
通讯作者：
Van Meurs, Krisa