Physiological and transcriptomic analyses characterized high temperature stress response mechanisms in Sorbus pohuashanensis.
Physiological and transcriptomic analyses characterized high temperature stress response mechanisms in Sorbus pohuashanensis.
复制标题
生理学和转录组学分析表征花椒高温胁迫响应机制
DOI：
10.1038/s41598-021-89418-7
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发表时间：
2021-05-12
影响因子：
4.6
通讯作者：
Zheng J
中科院分区：
文献类型：
--
作者：
Pei X;Zhang Y;Zhu L;Zhao D;Lu Y;Zheng J
花楸是我国特有的高山树种，但引种的问题还有待解决。坡华山松向低海拔地区转移的问题尚未得到解决。本研究的目的是探索S.利用RNA-Sequencing技术和生理生化测定方法研究了坡华山松对高温胁迫的响应。基于转录组数据，我们获得了1221个在8h 43℃处理期间差异表达的基因（752个上调，469个下调），候选基因与钙信号途径、植物激素信号传导、热激因子、伴侣蛋白、遍在蛋白介导的蛋白质水解、细胞壁修饰、活性氧清除酶、解毒和能量代谢相关。从生理水平和生化水平上分析了高温反应。叶片细胞叶绿素荧光参数降低，渗透调节物质含量增加，活性氧清除酶活性降低。S.本研究初步揭示了花楸属植物对高温胁迫的反应，为改进花楸属植物的引种方法、发掘花楸属植物与高温胁迫相关的耐热基因提供了基础信息，也为花楸属其他植物的高温胁迫研究提供了参考。
Sorbus pohuashanensis (Hance) Hedl. is a Chinese native alpine tree species, but the problem of introducing S. pohuashanensis to low altitude areas has not been solved. In this study, we aimed to explore the molecular regulatory network of S. pohuashanensis in response to high-temperature stress using RNA-Sequencing technology and physiological and biochemical determination. Based on transcriptomic data, we obtained 1221 genes (752 up-regulated and 469 down-regulated) that were differentially expressed during 8 h 43℃ treatment and candidate genes were related to calcium signaling pathway, plant hormone signal transduction, heat shock factors, chaperones, ubiquitin mediated proteolysis, cell wall modification, ROS scavenging enzymes, detoxification and energy metabolism. The analysis of high temperature response at the physiological level and biochemical level were performed. The chlorophyll fluorescence parameters of leaf cells decreased, the content of osmotic regulators increased, and the activity of ROS scavenging enzymes decreased. The molecular regulatory network of S. pohuashanensis in response to high-temperature stress was preliminarily revealed in this study, which provides fundamental information improving introducing methods and discovering heat-tolerant genes involved in high-temperature stress in this species and provides a reference for other plants of the genus Sorbus.
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影响因子：
14.9
作者：
Kanehisa M;Furumichi M;Sato Y;Ishiguro-Watanabe M;Tanabe M
通讯作者：
Tanabe M
影响因子：
5.6
作者：
Driedonks N;Xu J;Peters JL;Park S;Rieu I
通讯作者：
Rieu I
影响因子：
4.5
作者：
Khan, Adil;Bilal, Saqib;Lee, In-Jung
通讯作者：
Lee, In-Jung
影响因子：
2.1
作者：
Chen, Na;Yang, Qingli;Yu, Shanlin
通讯作者：
Yu, Shanlin
DOI：
10.1016/j.bbrc.2010.09.038
发表时间：
2010-10-15
影响因子：
3.1
作者：
Chen, Huan;Hwang, Jung Eun;Lim, Chae Oh
通讯作者：
Lim, Chae Oh