The Combination of Electroacupuncture and Massage Therapy Alleviates Myofibroblast Transdifferentiation and Extracellular Matrix Production in Blunt Trauma-Induced Skeletal Muscle Fibrosis.
The Combination of Electroacupuncture and Massage Therapy Alleviates Myofibroblast Transdifferentiation and Extracellular Matrix Production in Blunt Trauma-Induced Skeletal Muscle Fibrosis.
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DOI：
10.1155/2021/5543468
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Wang JG
中科院分区：
文献类型：
--
作者：
Zhao N;Liu B;Liu SW;Zhang W;Li HN;Pang G;Luo XF;Wang JG
补充疗法，如针灸和按摩，以前曾报道对骨骼肌挫伤有治疗效果。然而，电针联合按摩对钝性损伤后骨骼肌的恢复机制尚不清楚。本研究建立大鼠骨骼肌钝性损伤后骨骼肌纤维化模型，探讨EA +按摩治疗骨骼肌纤维化的可能分子机制。结果提示，与模型组比较，EA +按摩治疗可显著降低大鼠炎性细胞浸润和胶原纤维含量，改善肌原纤维内肌节紊乱。进一步分析发现，EA +按摩疗法可通过下调转化生长因子- (TGF-) β1和结缔组织生长因子（CTGF） mRNA和蛋白的表达，降低纤维化程度，增加肌成纤维细胞凋亡程度。此外，通过基质金属蛋白酶- (MMP-) 1和基质金属蛋白酶组织抑制剂（TIMP）平衡的正常化，治疗后损伤骨骼肌的纤维化得到抑制。上述结果提示，电针与按摩联合治疗可通过调节TGF β1- ctgf诱导的肌成纤维细胞转分化和细胞外基质生成的MMP-1/TIMP-1平衡来缓解纤维化过程。
Complementary therapies, such as acupuncture and massage, had been previously reported to have therapeutic effects on skeletal muscle contusions. However, the recovery mechanisms on skeletal muscles after blunt trauma via the combination of electroacupuncture (EA) and massage therapy remain unclear. In the present study, a rat model of the skeletal muscle fibrosis following blunt trauma to rat skeletal muscle was established, and the potential molecular mechanisms of EA + massage therapy on the skeletal muscle fibrosis were investigated. The results suggested that EA + massage therapy could significantly decrease inflammatory cells infiltration and collagenous fiber content and ameliorate the disarrangement of sarcomeres within myofibrils compared to the model group. Further analysis revealed that EA + massage therapy could reduce the degree of fibrosis and increase the degree of myofibroblast apoptosis by downregulating the mRNA and protein expression of transforming growth factor- (TGF-) β1 and connective tissue growth factor (CTGF). Furthermore, the fibrosis of injured skeletal muscle was inhibited after treatment through the normalization of balance between matrix metalloproteinase- (MMP-) 1 and tissue inhibitor of matrix metalloproteinase (TIMP). These findings suggested that the combination of electroacupuncture and massage therapy could alleviate the fibrotic process by regulating TGF β1-CTGF-induced myofibroblast transdifferentiation and MMP-1/TIMP-1 balance for extracellular matrix production.
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影响因子：
3
作者：
Baum J;Duffy HS
通讯作者：
Duffy HS
影响因子：
5
作者：
Holm Nielsen S;Willumsen N;Leeming DJ;Daniels SJ;Brix S;Karsdal MA;Genovese F;Nielsen MJ
通讯作者：
Nielsen MJ
影响因子：
3.3
作者：
Delos D;Maak TG;Rodeo SA
通讯作者：
Rodeo SA
DOI：
10.3109/08977194.2011.595714
发表时间：
2011-10
期刊：
Growth factors (Chur, Switzerland)
影响因子：
--
作者：
Biernacka A;Dobaczewski M;Frangogiannis NG
通讯作者：
Frangogiannis NG
影响因子：
17.1
作者：
Crane, Justin D.;Ogborn, Daniel I.;Tarnopolsky, Mark A.
通讯作者：
Tarnopolsky, Mark A.