Scatter factor expression and regulation in human glial tumors
Scatter factor expression and regulation in human glial tumors
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人胶质瘤中散射因子的表达和调控
DOI：
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发表时间：
1996
影响因子：
6.4
通讯作者：
I. Goldberg
中科院分区：
文献类型：
--
作者：
E. Rosen;J. Laterra;A. Joseph;L. Jin;A. Fuchs;D. Way;Marlys H. Witte;M. Weinand;I. Goldberg
散点因子（SF）又称肝细胞生长因子（HGF），是一种在体外诱导细胞运动和体内诱导血管生成的细胞因子。SF似乎是某些系统性癌症恶性表型的决定因素。我们在人脑胶质瘤提取液中检测到SF，其中在恶性肿瘤中含量最高。免疫组织化学证实，人胶质母细胞瘤细胞在体内同时表达SF及其受体（c - met蛋白）。与这些观察结果一致，我们发现培养的人胶质母细胞瘤细胞（8个系中的3个）和神经微血管内皮细胞（3个系中的3个）产生中高水平的免疫反应性和生物活性SF。SF通过旁分泌或自分泌机制刺激胶质母细胞瘤和NMVEC细胞系的增殖。来自胶质母细胞瘤和NMVEC细胞的条件培养基（CM）都含有SF诱导因子（SF - IF）活性，通过其刺激指示细胞系（MRC5人成纤维细胞）中SF生成的能力来定义。该活性由高分子量（约30 kDa）的热敏组分和低分子量（<30 kDa）的热稳定组分组成。此外，胶质母细胞瘤CM刺激了NMVEC SF的产生，而NMVEC CM刺激胶质母细胞瘤细胞SF的产生，在每种情况下都是3 - 6倍。我们的研究结果表明，胶质瘤细胞之间以及胶质瘤细胞与内皮之间的SF依赖性相互作用可以促进体内恶性胶质瘤的异质性增殖和血管生成表型。©1996 Wiley‐Liss, Inc。
Scatter factor (SF) (also known as hepatocyte growth factor [HGF]) is a cytokine that induces cell motility in vitro and angiogenesis in vivo. SF appears to be a determinant of the malignant phenotype in certain systemic cancers. We detected SF in extracts prepared from human gliomas, with the highest levels found in malignant tumors. Human glioblastoma cells expressed both SF and its receptor (c‐met protein) in vivo, as demonstrated by immunohistochemistry. Consistent with these observations, we found moderate to high levels of production of immunoreactive and biologically active SF by cultured human glioblastoma cells (3 of 8 lines) and by neural microvascular endothelial cells (NMVEC) (3 of 3 lines). SF stimulated the proliferation of glioblastoma and NMVEC cell lines by paracrine or autocrine mechanisms. Conditioned medium (CM) from both glioblastoma and NMVEC cells contained SF‐inducing factor (SF‐IF) activity, defined by its ability to stimulate SF production in an indicator cell line (MRC5 human fibroblasts). This activity consisted of a high‐molecular‐weight (>30 kDa), heat‐sensitive component and a low‐molecular weight (<30 kDa), heat‐stable component. Furthermore, glioblastoma CM stimulated NMVEC SF production, and NMVEC CM stimulated glioblastoma cell SF production, by 3‐ to 6‐fold in each case. Our findings demonstrate that SF‐dependent interactions between glioma cells, and between glioma cells and endothelium, can contribute to the heterogeneous proliferative and angiogenic phenotypes of malignant gliomas in vivo. © 1996 Wiley‐Liss, Inc.
影响因子：
11.2
作者：
J. Yamashita;M. Ogawa;S. Yamashita;K. Nomura;M. Kuramoto;T. Saishoji;Sadahito Shin
通讯作者：
J. Yamashita;M. Ogawa;S. Yamashita;K. Nomura;M. Kuramoto;T. Saishoji;Sadahito Shin
表达胰岛素样生长因子 1 (IGF-1) 受体反义 RNA 的大鼠胶质母细胞瘤细胞不具有致瘤性，可诱导野生型肿瘤消退。
影响因子：
11.2
作者：
Resnicoff,M;Sell,C;Rubini,M;Coppola,D;Ambrose,D;Baserga,R;Rubin,R
通讯作者：
Rubin,R
DOI：
10.1073/pnas.90.5.1937
发表时间：
1993-03-01
影响因子：
11.1
作者：
GRANT, DS;KLEINMAN, HK;ROSEN, EM
通讯作者：
ROSEN, EM