Enhanced glutamate uptake into synaptic vesicles fueled by vesicle-generated ATP from phosphoenolpyruvate and ADP. Proposed role of a novel enzyme.
Enhanced glutamate uptake into synaptic vesicles fueled by vesicle-generated ATP from phosphoenolpyruvate and ADP. Proposed role of a novel enzyme.
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DOI：
10.1007/s11064-012-0864-4
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发表时间：
2012-12
影响因子：
4.4
通讯作者：
Ueda, Tetsufumi
中科院分区：
文献类型：
--
作者：
Takeda, Kouji;Ueda, Tetsufumi
糖酵解ATP合成突触囊泡提供了一个有效的机制，燃料囊泡加载的神经递质谷氨酸。这部分是通过囊泡结合的丙酮酸激酶实现的。然而，我们已经发现，囊泡谷氨酸摄取，在丙酮酸激酶底物ADP和磷酸烯醇丙酮酸（PEP）的存在下，大大超过了外源性ATP所引起的。我们认为，这种增强的摄取部分是由于额外的ATP产生的机制，涉及一种新的酶，PEP依赖的ADP合酶。我们讨论了这种酶在能量稳态和病理生理学，以及在有效的突触谷氨酸传输的影响。
Glycolytic ATP synthesis by synaptic vesicles provides an efficient mechanism for fueling vesicular loading of the neurotransmitter glutamate. This is achieved in part by vesicle-bound pyruvate kinase. However, we have found that vesicular glutamate uptake, in the presence of the pyruvate kinase substrates ADP and phosphoenolpyruvate (PEP), substantially exceeds that caused by exogenous ATP. We propose that this much enhanced uptake is in part due to extra ATP produced via a mechanism involving a novel enzyme, PEP-dependent ADP synthase. We discuss implications for this enzyme in energy homeostasis and pathophysiology, as well as in efficient synaptic glutamate transmission.
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