ParA-like protein influences the distribution of multi-copy chromosomes in cyanobacterium Synechococcus elongatus PCC 7942.
ParA-like protein influences the distribution of multi-copy chromosomes in cyanobacterium Synechococcus elongatus PCC 7942.
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ParA 样蛋白影响蓝藻细长聚球藻 PCC 7942 中多拷贝染色体的分布。
DOI：
10.1099/mic.0.000577
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发表时间：
2018
期刊：
影响因子：
1.5
通讯作者：
Yoshikawa H
中科院分区：
文献类型：
--
作者：
Watanabe S;Noda A;Ohbayashi R;Uchioke K;Kurihara A;Nakatake S;Morioka S;Kanesaki Y;Chibazakura T;Yoshikawa H
虽然许多细菌，如大肠杆菌和枯草芽孢杆菌，拥有单拷贝染色体，但淡水蓝藻在每个细胞中都有多条染色体。虽然有报道称，在蓝细菌长聚球菌pcc 7942中，多拷贝染色体沿细胞长轴均匀分布，但这些染色体的分布机制尚不清楚。InS。长形体是一种代谢微室，用于固定碳，其分布方式与多拷贝染色体相似，由类ParA蛋白（ParA）调节。为了阐明ParA在多拷贝染色体分布中的作用，我们构建并分析了s的ParA干扰菌株和过表达菌株。elongatus。我们的荧光原位杂交实验显示，paradisruptants显示其多拷贝染色体的异常分布。在paradisruptant中，与每个染色体区域的细胞内位置相对应的多个原点和终点聚集在一起，这被来自其他基因组位点的外源ParA的表达所补偿。与WT菌株相比，paradisruptant对UV-C敏感。此外，在生长后期出现了ParA过表达的巨细胞，表明过量的ParA间接抑制了细胞分裂。通过酵母双杂交分析筛选para -相互作用蛋白，发现了4个参与DNA修复和细胞膜生物发生的候选蛋白。这些结果表明，ParA与这些蛋白一起参与了细胞的多效性功能，而ParA对细胞的生存能力则是必不可少的。elongatus。
While many bacteria, such asEscherichia coliandBacillus subtilis, harbour a single-copy chromosome, freshwater cyanobacteria have multiple copies of each chromosome per cell. Although it has been reported that multi-copy chromosomes are evenly distributed along the major axis of the cell in cyanobacteriumSynechococcus elongatusPCC 7942, the distribution mechanism of these chromosomes remains unclear. InS. elongatus, the carboxysome, a metabolic microcompartment for carbon fixation that is distributed in a similar manner to the multi-copy chromosomes, is regulated by ParA-like protein (hereafter ParA). To elucidate the role of ParA in the distribution of multi-copy chromosomes, we constructed and analysed ParA disruptant and overexpressing strains ofS. elongatus. Our fluorescencein situhybridization assay revealed that theparAdisruptants displayed an aberrant distribution of their multi-copy chromosomes. In theparAdisruptant the multiple origin and terminus foci, corresponding to the intracellular position of each chromosomal region, were aggregated, which was compensated by the expression of exogenous ParA from other genomic loci. TheparAdisruptant is sensitive to UV-C compared to the WT strain. Additionally, giant cells appeared under ParA overexpression at the late stage of growth indicating that excess ParA indirectly inhibits cell division. Screening of the ParA-interacting proteins by yeast two-hybrid analysis revealed four candidates that are involved in DNA repair and cell membrane biogenesis. These results suggest that ParA is involved in the pleiotropic cellular functions with these proteins, whileparAis dispensable for cell viability inS. elongatus.
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影响因子：
3.6
作者：
G. Real;S. Autret;E. Harry;J. Errington;A. Henriques
通讯作者：
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影响因子：
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作者：
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作者：
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Graumann, PL
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2006
影响因子：
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作者：
S. R. Thompson;G. Wadhams;J. Armitage
通讯作者：
J. Armitage
影响因子：
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作者：
Eckert, Carrie;Boehm, Marko;Maness, Pin-Ching
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