Use of baculovirus expression system for generation of virus-like particles: successes and challenges.
Use of baculovirus expression system for generation of virus-like particles: successes and challenges.
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DOI：
10.1016/j.pep.2013.05.009
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发表时间：
2013-08
影响因子：
1.6
通讯作者：
Wang Z
中科院分区：
文献类型：
--
作者：
Liu F;Wu X;Li L;Liu Z;Wang Z
简要介绍了北京谱仪的原理和应用。使用BES产生VLP。北京谱仪的主要性质：促进VLPs的产生。产生VLP的生物过程考虑因素。杆状病毒表达系统（baculovirus expression system，BES）是生产重组蛋白的通用平台之一，它需要进行多种翻译后修饰，如折叠、寡聚化、磷酸化、糖基化、酰化、二硫键形成和蛋白水解切割。重组DNA技术的进步促进了BES的应用，并使得在一次感染中同时表达多种蛋白质以及产生与其天然类似物具有功能相似性的多聚体蛋白质成为可能。因此，BES已被用于生产重组蛋白和构建病毒样颗粒（VLP），以及开发亚单位疫苗，包括基于VLP的疫苗。VLP由一个或多个结构蛋白组成，但没有病毒基因组，类似于真正的病毒体，但不能在细胞中复制。BES获得的高质量重组蛋白表达和翻译后修饰，沿着其产生多种蛋白的能力，意味着它理想地适合于VLP生产。在这篇文章中，我们批判性地回顾了使用BES作为平台来生产包封和非包封VLP的优点和缺点。
A brief overview of principles and applications of BES. Generation of VLPs using BES. Major properties of BES: promoting generation of VLPs. Bioprocess considerations for generation of VLPs. The baculovirus expression system (BES) has been one of the versatile platforms for the production of recombinant proteins requiring multiple post-translational modifications, such as folding, oligomerization, phosphorylation, glycosylation, acylation, disulfide bond formation and proteolytic cleavage. Advances in recombinant DNA technology have facilitated application of the BES, and made it possible to express multiple proteins simultaneously in a single infection and to produce multimeric proteins sharing functional similarity with their natural analogs. Therefore, the BES has been used for the production of recombinant proteins and the construction of virus-like particles (VLPs), as well as for the development of subunit vaccines, including VLP-based vaccines. The VLP, which consists of one or more structural proteins but no viral genome, resembles the authentic virion but cannot replicate in cells. The high-quality recombinant protein expression and post-translational modifications obtained with the BES, along with its capacity to produce multiple proteins, imply that it is ideally suited to VLP production. In this article, we critically review the pros and cons of using the BES as a platform to produce both enveloped and non-enveloped VLPs.
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影响因子：
13.8
作者：
Bieniossek C;Imasaki T;Takagi Y;Berger I
通讯作者：
Berger I
影响因子：
4.1
作者：
Bundy, Bradley C.;Swartz, James R.
通讯作者：
Swartz, James R.
影响因子：
2.9
作者：
Cheng, XG;Cole, RN;Hart, GW
通讯作者：
Hart, GW
影响因子：
5.4
作者：
ADANG, MJ;MILLER, LK
通讯作者：
MILLER, LK
影响因子：
14.9
作者：
BELYAEV, AS;ROY, P
通讯作者：
ROY, P