A truncated peptide Sp-NPFin from the neuropeptide FII SpNPFII of Scylla paramamosain exhibiting potent antimicrobial activity
A truncated peptide Sp-NPFin from the neuropeptide FII SpNPFII of Scylla paramamosain exhibiting potent antimicrobial activity
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来自准青蟹神经肽 FII SpNPFII 的截短肽 Sp-NPFin 表现出有效的抗菌活性
DOI：
10.1016/j.aquaculture.2020.736145
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发表时间：
2020-11
期刊：
影响因子：
4.5
通讯作者：
Wang Ke-Jian
中科院分区：
文献类型：
--
作者：
Zhang Hua;Yang Ying;Zhu Xuewu;Liu Yangzhou;Chen Fangyi;Wang Ke-Jian
神经肽（NP）F是脊椎动物神经肽Y的同源物，越来越多的证据表明，它除了在无脊椎动物神经生物学中发挥作用外，还可能在先天免疫中发挥作用。然而，到目前为止，关于甲壳类纳米颗粒的免疫学特性的报道很少。本研究从拟穴青蟹中克隆了一个神经肽基因，命名为SpNPFII。SpNPFII的cDNA全长为551 bp，编码124个氨基酸。结构预测表明，SpNPFII成熟肽主要形成α-螺旋结构。SpNPFII主要表达于雄蟹的性腺组织中，在输精管前部和精囊中的表达量最高。在脂多糖（LPS）和溶藻弧菌（Vibrio alginolyticus）刺激下，SpNPFII的表达水平发生显著变化。经过生物信息学分析，从SpNPFII中鉴定出一个截短的45个氨基酸的肽段，并将其命名为Sp-NPFin。Sp-NPFin具有很强的广谱抗菌活性（最小抑菌浓度[MIC]范围为1.5至12 μM）和抗霉菌活性（MIC范围为1.5至48 μM），低杀真菌浓度（例如，对尖孢镰刀菌的最低杀菌浓度[MBC]为1.5 - 3 μM）和快速杀菌动力学（在1 - 6 h内杀死所有细菌）。Sp-NPFin可以与几种微生物表面组分（例如LPS、脂磷壁酸、肽聚糖和葡聚糖）结合，并以浓度依赖性方式诱导微生物膜的显著损伤和引起细胞内容物的渗漏。共聚焦显微镜表明，Sp-NPFin定位于微生物表面。此外，Sp-NPF in对原代培养的蟹血细胞和哺乳动物细胞均无细胞毒性，体内实验表明，Sp-NPF in能显著提高S.在V.溶藻菌综上所述，SpNPFII可能在S.拟穴蛙肽及其截短肽Sp-NPFin在水产养殖、兽医和医药等领域具有广阔的应用前景。
Neuropeptide (NP) F is a homolog of vertebrate neuropeptide Y, and mounting evidence suggests that it may play a role in innate immunity in addition to its function in invertebrate neurobiology. However, so far, there have been few reports on the immunological properties of crustacean NPs. Here, we cloned a neuropeptide FII gene, naming it SpNPFII, from the mud crabScylla paramamosain. The full-length cDNA sequence of SpNPFII was 551 bp, encoding 124 amino acids. Structural prediction suggested that the SpNPFII mature peptide mainly formed an α-helix. SpNPFII was predominately expressed in male crab gonadal tissues, with the highest expression in the anterior vas deferens and seminal vesicle. The expression level of SpNPFII changed significantly under challenge by lipopolysaccharide (LPS) or Vibrio alginolyticus. After bioinformatic analysis, a truncated 45-amino acid peptide derived from SpNPFII and having potent antimicrobial activity was identified and named Sp-NPFin. Sp-NPFin exerted strong, broad-spectrum antibacterial (minimum inhibitory concentration [MIC] ranged from 1.5 to 12 μM) and antimildew activity (MIC ranged from 1.5 to 48 μM), a low fungicidal concentration (e.g., minimum bactericidal concentration [MBC] for Fusarium oxysporum was 1.5 to 3 μM) and rapid bactericidal kinetic (killed all bacteria within 1 to 6 h). Sp-NPFin could bind to several microbial surface components (e.g.LPS, lipoteichoic acid, peptidoglycan and glucan), and induce significant damage to microbial membranes and cause leakage of cell contents in a concentration-dependent manner. Confocal microscopy indicated that Sp-NPFin was localized at microbial surfaces. In addition, Sp-NPFin exhibited no cytotoxicity to primary cultured crab hemocytes and mammalian cells, andin vivostudy demonstrated that it could significantly improve the survival rate ofS. paramamosainunder the challenge ofV. alginolyticus. Taken together, SpNPFII might play a role in the immune system ofS. paramamosain, and its truncated peptide, Sp-NPFin, would be a promising antibacterial and antimildew agent with broad application prospects in the fields of aquaculture, veterinary practices and medicine.
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影响因子：
10.3
作者：
Sharma, Rishi;Sahota, Pradeep;Thakkar, Mahesh M.
通讯作者：
Thakkar, Mahesh M.
影响因子：
2.8
作者：
Augustyniak D;Nowak J;Lundy FT
通讯作者：
Lundy FT
DOI：
10.1007/s11626-013-9718-x
发表时间：
2014-05-01
影响因子：
2.1
作者：
Deepika, A.;Makesh, M.;Rajendran, K. V.
通讯作者：
Rajendran, K. V.
拟青蟹血细胞脂多糖差异表达基因的鉴定
影响因子：
4.7
作者：
Chen, Fang-Yi;Liu, Hai-Peng;Wang, Ke-Jian
通讯作者：
Wang, Ke-Jian
影响因子：
5.2
作者：
K. Matsuda;Ki Sung Kang;Atsushi Sakashita;S. Yahashi;H. Vaudry
通讯作者：
K. Matsuda;Ki Sung Kang;Atsushi Sakashita;S. Yahashi;H. Vaudry