Active immunity and T-cell populations in pigs intraperitoneally inoculated with baculovirus-expressed transmissible gastroenteritis virus structural proteins.
Active immunity and T-cell populations in pigs intraperitoneally inoculated with baculovirus-expressed transmissible gastroenteritis virus structural proteins.
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DOI：
10.1016/s0165-2427(99)00074-4
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发表时间：
1999-09-20
影响因子：
1.8
通讯作者：
Saif LJ
中科院分区：
文献类型：
--
作者：
Sestak K;Meister RK;Hayes JR;Kim L;Lewis PA;Myers G;Saif LJ
将杆状病毒表达的传染性胃肠炎病毒（TGEV）结构蛋白（S、N、M）与大肠杆菌不耐热突变毒素（LT-R192 G）混合，用不完全弗氏佐剂（IFA）腹腔接种猪，以诱导猪肠道相关淋巴组织（GALT）对TGEV的主动免疫。使用四组63头（1-5周龄）TGEV血清阴性哺乳猪评估重组蛋白疫苗（第3组）与假疫苗（第1组）、商业疫苗（第2组）和强毒TGEV Miller株接种猪（第4组）相比的效力。在体内和体外用TGEV抗原刺激后测量TGEV特异性粘膜和全身免疫应答。用TGEV刺激单核细胞（来自接种TGEV重组蛋白的第3组肠系膜淋巴结）后，在体内和体外分析主要T细胞亚群分布。通过在27日龄时用强毒TGEV攻击猪来评估主动免疫的诱导。杆状病毒表达的TGEV蛋白与LT-R192 G在IFA中共同施用诱导与TGEV的IgA抗体相关的肠系膜淋巴结免疫应答和针对TGEV攻击的部分保护。第3组猪血清IgG和病毒中和抗体的高滴度最可能反映了TGEV S蛋白的给药剂量。在TGEV攻击当天，用灭活的TGEV体外刺激来自第3组猪的肠系膜淋巴结的单核细胞，导致双阳性细胞增加。（CD 4 + CD 8+），自然杀伤细胞（CD 2 + CD 4 − CD 8 +dim）和细胞毒性（CD 2 + CD 4 − CD 8+亮）T细胞表型，伴随白细胞介素-2受体表达增加和无效（CD 2 − CD 4 − CD 8 −/SW 6+）细胞表型减少。
The intraperitoneal inoculation of pigs with baculovirus-expressed transmissible gastroenteritis virus (TGEV) structural proteins (S, N, M) in conjunction with thermolabile Escherichia coli mutant toxin (LT-R192G) in incomplete Freund’s adjuvant (IFA) was tested in an attempt to elicit active immunity to TGEV in gut-associated lymphoid tissues (GALT). Four groups of 63 (1–5-week-old) suckling, TGEV-seronegative pigs were used to assess the efficacy of the recombinant protein vaccine (group 3) in comparison with sham (group 1), commercial vaccine (group 2), and virulent TGEV Miller-strain-inoculated pigs (group 4). The TGEV-specific mucosal and systemic immune responses were measured after in vivo and in vitro stimulation with TGEV-antigens. The major T-cell subset distribution was analyzed in vivo and in vitro after stimulation of mononuclear cells with TGEV (from mesenteric lymph nodes of group 3 inoculated with TGEV-recombinant proteins). Induction of active immunity was assessed by challenge of pigs with virulent TGEV at 27 days of age. Baculovirus-expressed TGEV proteins coadministered with LT-R192G in IFA induced mesenteric lymph node immune responses associated with IgA-antibodies to TGEV and partial protection against TGEV-challenge. The high titers of serum IgG- and virus-neutralizing-antibodies to TGEV in group 3 pigs most likely reflected the dose of TGEV S-protein administered. At the day of TGEV-challenge, the in vitro stimulation of mononuclear cells from the mesenteric lymph nodes of group 3 pigs with inactivated TGEV resulted in an increase in double positive (CD4+CD8+), natural killer (CD2+CD4−CD8+dim) and cytotoxic (CD2+CD4−CD8+bright) T-cell phenotypes, accompanied by increased expression of interleukin-2 receptor and a decrease of the null (CD2−CD4−CD8−/SW6+) cell phenotype.
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