Extraction and identification of the chyme proteins in the digestive tract of growing pigs
Extraction and identification of the chyme proteins in the digestive tract of growing pigs
复制标题
生长猪消化道食糜蛋白的提取及鉴定
DOI：
10.1007/s11427-017-9356-1
复制
发表时间：
2018-08
期刊：
影响因子：
--
通讯作者：
Yin Yulong
中科院分区：
文献类型：
--
作者：
Li Jianjun;Tan Bie;Tang Yulong;Liao Peng;Yao Kang;Ji Peng;Yin Yulong
This study aimed to explore the rule of degradation of dietary proteins by identifying chyme proteins in different segments of the digestive tract of growing pigs, using proteomics techniques. Six growing pigs were fed a corn-soybean meal-based diet for 7 days. The feedstuff and chyme proteins were separately extracted and separated with SDS-PAGE. 2D LCMS/MS combined with protein database searching identified 1,513 proteins in different segments of the gastrointestinal tract, the number of identified exogenous proteins gradually decline from the stomach to colon, with large amounts in the duodenum to the large intestine. More corn proteins than soybean proteins were identified both in the feedstuff and chyme, and these were significantly decreased after digestion in the stomach. More membrane proteins than non-membrane proteins were identified in whole digestive tract. These results regarding the profiles of chyme proteins in different segments of the gastrointestinal tract would provide useful information for optimizing feed formula in pigs.
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影响因子：
3.3
作者：
C. F. M. Lange;W. Souffrant;W. Sauer
通讯作者：
C. F. M. Lange;W. Souffrant;W. Sauer
DOI：
10.1093/jn/136.7.1759
发表时间：
2006-07
期刊：
The Journal of nutrition
影响因子：
--
作者：
Junjun Wang;Defa Li;L. Dangott;Guoyao Wu
通讯作者：
Junjun Wang;Defa Li;L. Dangott;Guoyao Wu
影响因子：
3.3
作者：
H. Schulze;P. Leeuwen;M. Verstegen;J.W.O. van den Berg
通讯作者：
H. Schulze;P. Leeuwen;M. Verstegen;J.W.O. van den Berg
影响因子：
3.5
作者：
Rezaei, Reza;Knabe, Darrell A.;Wu, Guoyao
通讯作者：
Wu, Guoyao
影响因子：
4.2
作者：
ASCHE, GL;LEWIS, AJ;PEO, ER
通讯作者：
PEO, ER