Metabolite profiling reveals organ-specific flavone accumulation in Scutellaria and identifies a scutellarin isomer isoscutellarein 8-O-β-glucuronopyranoside.
Metabolite profiling reveals organ-specific flavone accumulation in Scutellaria and identifies a scutellarin isomer isoscutellarein 8-O-β-glucuronopyranoside.
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作者：
Askey BC;Liu D;Rubin GM;Kunik AR;Song YH;Ding Y;Kim J
紫茎泽兰是一种含有多个物种的植物属，具有良好的药用效果。 S. baicalensis和S. Barbata是研究得最好的紫藤属物种之一，以前的工作已经确定黄酮是其生物活性的主要来源。最近的基因组和生化研究与S。baicalensis和S. barbata等的研究，提高了我们对紫藤属植物黄酮生物合成的认识。然而，由于世界各地有数百种紫藤属植物，大多数物种的黄酮生物合成仍然知之甚少。在这项研究中，我们分析了七种紫藤属植物的器官特异性黄酮谱，包括S。baicalensis、S. barbata，和两个物种原产于美洲（S。wrightii到Texas和S.总状花序到中美洲和南美洲）。我们发现，几乎所有这些物种的根只产生4′-脱氧黄酮，而4′-羟基黄酮只积累在其地上部分。另一方面，S. racemosa和S. wrightii在地上部分也积累了大量的4′-脱氧黄酮，这与S. baicalensis和S.巴巴塔此外，我们的代谢组学和NMR研究确定了异黄芩素8-O-β-葡萄糖醛酸吡喃糖苷（一种罕见的4′-羟基黄酮）在几种紫藤属植物（包括S. baicalensis和S. barbata，而S. racemosa和S.赖特紫藤属物种中独特的器官特异性代谢产物谱表明黄酮的选择性和不同的生理作用。
Scutellaria is a genus of plants containing multiple species with well‐documented medicinal effects. S. baicalensis and S. barbata are among the best‐studied Scutellaria species, and previous works have established flavones to be the primary source of their bioactivity. Recent genomic and biochemical studies with S. baicalensis and S. barbata have advanced our understanding of flavone biosynthesis in Scutellaria. However, as over several hundreds of Scutellaria species occur throughout the world, flavone biosynthesis in most species remains poorly understood. In this study, we analyzed organ‐specific flavone profiles of seven Scutellaria species, including S. baicalensis , S. barbata , and two species native to the Americas ( S. wrightii to Texas and S. racemosa to Central and South America). We found that the roots of almost all these species produce only 4′‐deoxyflavones, while 4′‐hydroxyflavones are accumulated exclusively in their aerial parts. On the other hand, S. racemosa and S. wrightii also accumulated high levels of 4′‐deoxyflavones in their aerial parts, different with the flavone profiles of S. baicalensis and S. barbata . Furthermore, our metabolomics and NMR study identified the accumulation of isoscutellarein 8‐O‐β‐glucuronopyranoside, a rare 4′‐hydroxyflavone, in the stems and leaves of several Scutellaria species including S. baicalensis and S. barbata , but not in S. racemosa and S. wrightii . Distinctive organ‐specific metabolite profiles among Scutellaria species indicate the selectivity and diverse physiological roles of flavones.
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影响因子：
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作者：
Tsai CC;Lin CS;Hsu CR;Chang CM;Chang IW;Lin LW;Hung CH;Wang JL
通讯作者：
Wang JL
影响因子：
3.6
作者：
Jiang, Zhenzuo;Yang, Jing;Jiao, Yujiao;Li, Wayne;Chai, Xin;Zhang, Lei;Jiang, Miaomiao;Wang, Yuefei
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Wang, Yuefei
影响因子：
4.6
作者：
Gallon, Marilia Elias;Silva-Junior, Eduardo Afonso;Gobbo-Neto, Leonardo
通讯作者：
Gobbo-Neto, Leonardo
黄芩和半枝莲的比较基因组分析揭示活性黄酮生物合成的进化
DOI：
10.1016/j.gpb.2020.06.002
发表时间：
2020-06
期刊：
Genomics, proteomics & bioinformatics
影响因子：
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作者：
Xu Z;Gao R;Pu X;Xu R;Wang J;Zheng S;Zeng Y;Chen J;He C;Song J
通讯作者：
Song J
影响因子：
5.6
作者：
Slámová K;Kapešová J;Valentová K
通讯作者：
Valentová K