Galega officinalis extract regulate the diabetes mellitus related violations of proliferation, functions and apoptosis of leukocytes.
Galega officinalis extract regulate the diabetes mellitus related violations of proliferation, functions and apoptosis of leukocytes.
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DOI：
10.1186/s12906-017-2079-3
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发表时间：
2018-01-08
影响因子：
--
通讯作者：
Sybirna N
中科院分区：
文献类型：
--
作者：
Nagalievska M;Sabadashka M;Hachkova H;Sybirna N
白细胞功能受损是导致糖尿病患者对感染易感的因素。了解糖尿病发病机制中涉及的免疫学过程的最重要的是给出疾病过程中免疫学特征及其变化。山羊豆是民间治疗糖尿病的常用药材。被用来纠正免疫系统功能失调实验在雄性Wistar大鼠上进行。在三层聚蔗糖-泛影酸钠密度梯度中进行骨髓细胞悬液的分级。用5-溴-2 ′-脱氧尿苷（BrdU）酶免疫分析法检测淋巴细胞-粒细胞增殖活性。对于骨髓制备物的染色，使用May-Gruenwald-Romanowsky-Giemsa（Pappenheim）方法。为评价嗜中性白细胞阳离子蛋白和髓过氧化物酶的含量，进行了细胞化学研究。免疫酶法测定肿瘤坏死因子α含量。用Annexin V荧光分析法检测淋巴细胞凋亡。糖尿病的发展伴随着中性粒细胞和淋巴细胞增殖的破坏、髓过氧化物酶活性的增加和凋亡过程的增强。在糖尿病条件下施用山羊豆提取物促进中性粒细胞骨髓池的恢复和淋巴母细胞数量的减少，并导致淋巴细胞凋亡过程的抑制。研究药物在糖尿病条件下具有显著的免疫校正作用，可以成为创造新一代抗糖尿病药物的基础。
An impaired leukocytes function is the factor causing the susceptibility of patients with diabetes mellitus to infections. The outmost importance for the understanding of the immunological processes involved in diabetes pathogenesis is to give the characteritics of the immunological profile and changes therein, during the course of desease. Long-used in folk medicine to treat diabetes Galega officinalis L. has been chosen for the correction of the immune system dysfunction. The experiments were conducted on male Wistar rats. Fractionation of bone marrow cells suspension was performed in a three-layer ficoll–sodium amidotrizoate density gradient. The lymphocytic-granulocytic cells proliferative activity was studied using enzyme immunoassay with 5-bromo-2′-deoxyuridine (BrdU). For staining of bone marrow preparations May-Gruenwald-Romanowsky-Giemsa (Pappenheim) method was used. To evaluate the content of cationic proteins and myeloperoxidase in neutrophilic leukocytes cytochemical studies were performed. Content of tumor necrosis factor alpha was carried out by immuno-enzymatic analysis. Lymphocytes apoptosis was examined by fluorescent analysis using annexin V. Diabetes mellitus development was accompanied with violation of neutrophils and lymphocytes proliferation, increased activity of myeloperoxidase and enhanced apoptosis process. Administration of Galega officinalis extract under the condition of diabetes promotes the restoration of neutrophils bone marrow pool and the reduction of lymphoblasts number and causes inhibition of the lymphocytes apoptosis process. Investigated medicine has a pronounced immunocorrective effect under the conditions of diabetes mellitus and can become the basis for creating a new generation of antidiabetic drugs.
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影响因子：
6.1
作者：
HIRANO, T;OKA, K;AKIBA, M
通讯作者：
AKIBA, M
影响因子：
--
作者：
Geerlings, SE;Hoepelman, AIM
通讯作者：
Hoepelman, AIM
影响因子：
7.1
作者：
Caughey, GE;Mantzioris, E;James, MJ
通讯作者：
James, MJ
影响因子：
5.6
作者：
Cardeno, Ana;Aparicio-Soto, Marina;Alarcon-de-la-Lastra, Catalina
通讯作者：
Alarcon-de-la-Lastra, Catalina
影响因子：
64.8
作者：
Fadok, VA;Bratton, DL;Henson, PM
通讯作者：
Henson, PM