EARLY FLOWERING3 Gene Confers Earlier Flowering and Enhancement of Salt Tolerance in Woody Plant Osmanthus fragrans
EARLY FLOWERING3 Gene Confers Earlier Flowering and Enhancement of Salt Tolerance in Woody Plant Osmanthus fragrans
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EARLY FLOWERING3基因赋予木本植物桂花提早开花和增强耐盐性
作者：
郑子飞;陈鹏伟;曹珊珊;钟诗蔚;王艺光;杨丽媛;方遒;肖政;赵宏波;董彬
桂花在亚洲的园林和园艺中很受欢迎。近年来，人们越来越关注万年青树木的开花和适应。早花蛋白3（Early Flowering 3，ELF 3）在植物开花调控和抗非生物胁迫中起重要作用。然而，关于ELF 3基因如何影响O.香。为了阐明成花相关基因ELF 3在植物耐盐应答中的潜在作用，通过RNA测序（RNA-seq）获得了盐胁迫下成花相关基因OfELF 3的表达上调基因。香。我们的研究结果表明OfELF 3是一个核蛋白，它不具有转录激活能力。qRT-PCR检测了OfELF 3基因在不同组织和花芽分化过程中的积累，盐胁迫处理也显著诱导了OfELF 3基因的表达。此外，OfELF 3的过表达加速了转基因拟南芥系的开花时间，并且研究了开花整合子如AtFT、AtSOC 1和AtAP 1的表达的增加。此外，OfELF 3过表达显著提高了转基因植株的耐盐性，在萌发后4 d和幼苗5 d的NaCl处理下，转基因植株的种子萌发率和根长均上级优于野生型（WT）。3周龄转基因植株的表型和生理指标（REL、MDA和可溶性蛋白）也明显优于野生型植株（上级）。综上所述，我们的研究结果表明OfELF 3不仅是一个正调节因子，在开花的调节，但也参与了耐盐性反应在O。香。
Osmanthus fragrans Lour. is popular in landscaping and gardening in Asia. In recent years, growing attention has been given to evergreen tree flowering and adaptation. EARLY FLOWERING3 (ELF3) plays an essential role in plant flowering regulation and abiotic stress tolerance. However, there is very little known about how the ELF3 gene affects flowering time and salt tolerance in O. fragrans. To elucidate the potential role of the flowering-related gene ELF3 in responding to salt tolerance, a significantly upregulated gene OfELF3 was obtained by RNA sequencing (RNA-seq) after salt treatment in O. fragrans. Our results showed that OfELF3 is a nuclear protein, which did not have a transcriptional activation ability. OfELF3 accumulation was determined in different tissues and the differentiation process of floral buds by qRT–PCR, and the gene was also significantly induced by salt stress treatment. In addition, overexpression of OfELF3 accelerated the flowering time of transgenic Arabidopsis lines, and an increase in the expression of flowering integrators such as AtFT, AtSOC1, and AtAP1 was investigated. Moreover, OfELF3 overexpression significantly improved the salt tolerance of transgenic plants, seed germination and root length of transgenic plants and was superior to those of the wild type (WT) under NaCl treatment at 4 days post-germination and the 5-day-old seedling stage, respectively. Similarly, phenotype and physiological indexes (REL, MDA and soluble protein) of 3-week-old transgenic plants were superior to the WT plants as well. Together, our results suggest that OfELF3 is not only a positive regulator in the regulation of flowering but is also involved in the salt tolerance response in O. fragrans.
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影响因子：
16
作者：
Yu, Jae-Woong;Rubio, Vicente;Lee, Na-Yeoun;Bai, Sulan;Lee, Sun-Young;Kim, Sang-Sook;Liu, Lijing;Zhang, Yiyue;Irigoyen, Maria Luisa;Sullivan, James A.;Zhang, Yu;Lee, Ilha;Xie, Qi;Paek, Nam-Chon;Deng, Xing Wang
通讯作者：
Deng, Xing Wang
影响因子：
5.6
作者：
R. Zheng;Zhenyin Zhu;Yanli Wang;Shiyang Hu;W. Xi;Wei Xiao;Xiaolu Qu;Linlin Zhong;Q. Fu;Caiyun Wang
通讯作者：
R. Zheng;Zhenyin Zhu;Yanli Wang;Shiyang Hu;W. Xi;Wei Xiao;Xiaolu Qu;Linlin Zhong;Q. Fu;Caiyun Wang
桂花的 OfSPL11 基因促进拟南芥植物生长和减少氧化损伤，增强耐盐性
影响因子：
3.1
作者：
朱守阔;方遒;王艺光;钟诗蔚;董彬;赵宏波
通讯作者：
赵宏波
影响因子：
6.2
作者：
Wang, Ke;Zhong, Ming;Liu, Guang-li
通讯作者：
Liu, Guang-li
影响因子：
5.6
作者：
Shi, Wen-Yan;Du, Yong-Tao;Zhang, Xiao-Hong
通讯作者：
Zhang, Xiao-Hong