LIA5 is required for nuclear reorganization and programmed DNA rearrangements occurring during tetrahymena macronuclear differentiation.
LIA5 is required for nuclear reorganization and programmed DNA rearrangements occurring during tetrahymena macronuclear differentiation.
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DOI：
10.1371/journal.pone.0075337
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Chalker DL
中科院分区：
文献类型：
--
作者：
Shieh AW;Chalker DL
在大核分化的纤毛四膜虫嗜热，全基因组DNA重排消除近50 Mbp的生殖细胞衍生的DNA，创造一个精简的体细胞基因组。使用皮尔纳途径鉴定待消除的转座子样和其他重复序列，并在其去除之前包装为异染色质。在这项研究中，我们表明，LIA5，它编码一个锌指蛋白可能的转座子起源，是所需的染色体片段化和DNA消除事件。Lia5p在RNAi指导的异染色质修饰建立之后，但在修饰序列的切除之前起作用。在MALLIA 5细胞中，DNA消除灶，含有待消除序列的大的核亚结构和必需的染色体结构域蛋白Pdd1p，不形成。与Pdd1p不同，Lia5p与这些结构不稳定相关，但它们的形成需要Lia5p。在不存在Lia5p的情况下，我们可以通过UV处理异位诱导DNA损伤来恢复病灶形成。在野生型或UV处理过的ARMLIA5细胞中的病灶都含有去磷酸化的Pdd1p。LIA5的这些研究表明，DNA消除灶形成后发生生殖系有限的序列切除，并表明Pdd1p重组可能是通过DNA损伤反应介导的。
During macronuclear differentiation of the ciliate Tetrahymena thermophila, genome-wide DNA rearrangements eliminate nearly 50 Mbp of germline derived DNA, creating a streamlined somatic genome. The transposon-like and other repetitive sequences to be eliminated are identified using a piRNA pathway and packaged as heterochromatin prior to their removal. In this study, we show that LIA5, which encodes a zinc-finger protein likely of transposon origin, is required for both chromosome fragmentation and DNA elimination events. Lia5p acts after the establishment of RNAi-directed heterochromatin modifications, but prior to the excision of the modified sequences. In ∆LIA5 cells, DNA elimination foci, large nuclear sub-structures containing the sequences to be eliminated and the essential chromodomain protein Pdd1p, do not form. Lia5p, unlike Pdd1p, is not stably associated with these structures, but is required for their formation. In the absence of Lia5p, we could recover foci formation by ectopically inducing DNA damage by UV treatment. Foci in both wild-type or UV-treated ∆LIA5 cells contain dephosphorylated Pdd1p. These studies of LIA5 reveal that DNA elimination foci form after the excision of germ-line limited sequences occurs and indicate that Pdd1p reorganization is likely mediated through a DNA damage response.
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影响因子：
56.9
作者：
Celeste, A;Petersen, S;Nussenzweig, A
通讯作者：
Nussenzweig, A
DOI：
10.1083/jcb.200904033
发表时间：
2009-05-18
期刊：
The Journal of cell biology
影响因子：
--
作者：
Ball AR Jr;Yokomori K
通讯作者：
Yokomori K
在酵母和哺乳动物中，异染色质对 γ-H2AX 修饰具有抵抗力。
DOI：
10.1083/jcb.200612031
发表时间：
2007-07-16
期刊：
The Journal of cell biology
影响因子：
--
作者：
Kim JA;Kruhlak M;Dotiwala F;Nussenzweig A;Haber JE
通讯作者：
Haber JE
影响因子：
3.4
作者：
De Palmenaer, Daniel;Siguier, Patricia;Mahillon, Jacques
通讯作者：
Mahillon, Jacques
DOI：
10.1016/j.bbamcr.2008.07.012
发表时间：
2008-11
期刊：
Biochimica et biophysica acta
影响因子：
--
作者：
Chalker DL
通讯作者：
Chalker DL