Occurrence of Oxidative Stress and Premature Senescence in the Anterior Segment of Acute Primary Angle-Closure Eyes.
Occurrence of Oxidative Stress and Premature Senescence in the Anterior Segment of Acute Primary Angle-Closure Eyes.
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急性原发性闭角眼眼前段氧化应激及早衰的发生
DOI：
10.1167/iovs.63.1.34
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发表时间：
2022-01-03
影响因子：
4.4
通讯作者：
Huang J
中科院分区：
文献类型：
--
作者：
Ye D;Xu Y;Shi Y;Ji J;Lu X;Chen H;Huang R;Lu P;Li Y;Cheng L;Li Y;Cui K;Tang X;Luo L;Huang J
目的探讨急性原发性房角关闭（APAC）患者眼压升高后眼前节是否发生氧化应激和早衰。方法收集21例APAC患者、22例年龄相关性白内障患者和10例健康志愿者的眼标本。采集房水（AqH）、虹膜和前透镜囊样本。采用相关试剂盒和多重磁珠免疫分析技术检测AqH中氧化应激标志物和衰老相关分泌表型（SASP）相关细胞因子的水平。通过免疫荧光和衰老相关β-半乳糖苷酶（SA-β-gal）染色检测眼前节组织中相关标志物的强度。结果APAC眼AqH中氧化应激标志物水平显著升高。活性氧（ROS）和8-羟基脱氧鸟苷水平与术前峰值眼压和年龄呈正相关，而还原型谷胱甘肽/氧化型谷胱甘肽（GSH/GSSH）比值与这两个参数呈负相关。几种SASP相关细胞因子的水平显着增加。ROS和丙二醛水平与某些SASP相关细胞因子水平呈正相关，而超氧化物歧化酶水平和GSH/GSSH比值呈相反趋势。APAC组虹膜和前透镜囊中线粒体DNA氧化损伤和晶状体变性相关标志物阳性的细胞数量增加。APAC组虹膜中衰老相关标志物（p16、p21和p53）和SA-β-gal活性增加。结论APAC患者眼前节存在氧化应激和早衰现象，可能参与了APAC眼前节病变的发生发展。
Purpose To explore whether oxidative stress and premature senescence occur in the anterior segment of acute primary angle-closure (APAC) eyes after increased intraocular pressure. Methods The eye samples of 21 APAC patients, 22 age-related cataract patients, and 10 healthy donors were included. Aqueous humor (AqH), iris, and anterior lens capsule samples were collected. The levels of oxidative stress markers and senescence-associated secretory phenotype (SASP)–related cytokines in AqH were estimated using relevant reagent kits and multiplex bead immunoassay technique. The intensity of relevant markers in anterior segment tissues was examined by immunofluorescence- and senescence-associated β-galactosidase (SA-β-gal) staining. Results Oxidative stress marker levels elevated significantly in the AqH of APAC eyes. Reactive oxygen species (ROS) and 8-hydroxydeoxyguanosine levels were positively correlated with preoperative peak intraocular pressure and age, whereas reduced glutathione/oxidized glutathione (GSH/GSSH) ratio was negatively correlated with both parameters. The levels of several SASP-related cytokines were markedly increased. ROS and malondialdehyde levels were positively correlated with the levels of some SASP-related cytokines, whereas superoxide dismutase level and GSH/GSSH ratio showed an opposite trend. The number of cells positive for oxidative mitochondrial DNA damage and apoptosis-related markers increased in the iris and anterior lens capsule of the APAC group. Senescence-associated markers (p16, p21, and p53) and SA-β-gal activity were increased in the iris of the APAC group. Conclusions Oxidative stress and premature senescence occurred in the anterior segment of APAC patients, suggesting that they may be involved in the development of pathological changes in the anterior segment of APAC eyes.
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影响因子：
8.8
作者：
Baker DJ;Weaver RL;van Deursen JM
通讯作者：
van Deursen JM
影响因子：
6.7
作者：
Khanehzar A ;Fraire JC ;Xi M ;Feizpour A ;Xu F ;Wu L ;Coronado EA ;Reinhard BM
通讯作者：
Reinhard BM
影响因子：
4.4
作者：
Guzman, Celeste P.;Gong, Tianxia;Aung, Tin
通讯作者：
Aung, Tin
影响因子：
10.5
作者：
Ohanna, Mickael;Giuliano, Sandy;Bertolotto, Corine
通讯作者：
Bertolotto, Corine
慢性原发性闭角不同阶段患者虹膜胶原与体内眼前节结构的相关性
影响因子：
3.1
作者：
Chung, Chuchen;Dai, Miaomiao;Huang, Jingjing
通讯作者：
Huang, Jingjing