ENU-induced mutagenesis in grass carp (Ctenopharyngodon idellus) by treating mature sperm.
ENU-induced mutagenesis in grass carp (Ctenopharyngodon idellus) by treating mature sperm.
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DOI：
10.1371/journal.pone.0026475
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Zou SM
中科院分区：
文献类型：
--
作者：
Jiang XY;Sun CF;Zhang QG;Zou SM
N-乙基-N-亚硝基脲 (ENU) 诱变是植物遗传改良以及在小鼠和斑马鱼等动物模型中诱导功能突变体的有用方法。在本研究中，草鱼（Ctenopharyngodon idellus）的成熟精子用一定浓度的ENU处理45分钟，然后使野生型卵受精。结果表明，随着ENU剂量增加至10 mM，分节期形态异常的胚胎或孵化期死苗的比例增加。选择具有诱变性但能提供足够数量的存活鱼苗的剂量，从 1 mM ENU 处理的精子中产生 F1 群体用于筛选目的。 ENU 处理的 F1 群体在第一年的生长表现出巨大的变化。与对照相比，产生了一些形态正常的较大突变体。对 15 个 F1 ENU 处理个体的 DNA 分析 igf-2a、igf-2b、mstn-1、mstn-2、fst-1 和 fst-2 位点部分编码区的突变表明，大多数 ENU 处理的点突变是 GC 到 AT 或 AT 到 GC 替换，这导致无义、非同义和同义突变。检查位点的平均突变率为0.41%。这些结果表明，成熟精子的 ENU 处理可以有效诱导草鱼的点突变，这可能是这些鱼类遗传改良的潜在有用方法。
N-ethyl-N-nitrosourea (ENU) mutagenesis is a useful approach for genetic improvement of plants, as well as for inducing functional mutants in animal models including mice and zebrafish. In the present study, mature sperm of grass carp (Ctenopharyngodon idellus) were treated with a range of ENU concentrations for 45 min, and then wild-type eggs were fertilized. The results indicated that the proportion of embryos with morphological abnormalities at segmentation stage or dead fry at hatching stage increased with increasing ENU dose up to 10 mM. Choosing a dose that was mutagenic, but provided adequate numbers of viable fry, an F1 population was generated from 1 mM ENU-treated sperm for screening purposes. The ENU-treated F1 population showed large variations in growth during the first year. A few bigger mutants with morphologically normal were generated, as compared to the controls. Analysis of DNA from 15 F1 ENU-treated individuals for mutations in partial coding regions of igf-2a, igf-2b, mstn-1, mstn-2, fst-1and fst-2 loci revealed that most ENU-treated point mutations were GC to AT or AT to GC substitution, which led to nonsense, nonsynonymous and synonymous mutations. The average mutation rate at the examined loci was 0.41%. These results indicate that ENU treatment of mature sperm can efficiently induce point mutations in grass carp, which is a potentially useful approach for genetic improvement of these fish.
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影响因子：
14.9
作者：
Nickerson, DA;Tobe, VO;Taylor, SL
通讯作者：
Taylor, SL
影响因子：
4.5
作者：
Goda, Tadahiro;Abu-Daya, Anita;Zimmerman, Lyle B.
通讯作者：
Zimmerman, Lyle B.
影响因子：
--
作者：
Jin, P;Tian, T;Meng, AM
通讯作者：
Meng, AM
DOI：
10.1073/pnas.92.13.5997
发表时间：
1995-06-20
影响因子：
11.1
作者：
RILEY, BB;GRUNWALD, DJ
通讯作者：
GRUNWALD, DJ
影响因子：
4.5
作者：
Liu, Feng;Xia, Jun-Hong;Yue, Gen Hua
通讯作者：
Yue, Gen Hua