The complete sequence of the bovine torovirus genome.
The complete sequence of the bovine torovirus genome.
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DOI：
10.1016/j.virusres.2005.07.005
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发表时间：
2006-01
期刊：
影响因子：
5
通讯作者：
Tellier R
中科院分区：
文献类型：
--
作者：
Draker R;Roper RL;Petric M;Tellier R
冠状病毒科的病毒引起了新的兴趣，2003年SARS HCoV引起的爆发和最近发现的一种新的人类冠状病毒HCoV-NL 63。冠状病毒科中的圆环病毒属的特征不太清楚，部分原因是圆环病毒还不能在细胞培养中生长（伯尔尼病毒除外）。在这项研究中，我们确定了布雷达-1（BoTV-1），牛圆环病毒的全基因组序列。这是第一个完整的圆环病毒基因组序列被报道。使用长RT-PCR扩增BoTV-1 RNA，并对扩增子进行测序。基因组长度为28.475 kb，主要由复制酶基因（约20.2 kb）组成，该基因含有两个大的重叠ORF，ORF 1a和ORF 1b，分别编码多聚蛋白pp 1a和pp 1b。序列分析确定了pp 1a和pp 1b预测序列内的保守结构域。序列比对和蛋白质二级结构预测数据表明存在一个3C样丝氨酸蛋白酶结构域与动脉炎病毒3C样丝氨酸蛋白酶和一个单一的木瓜蛋白酶样半胱氨酸蛋白酶结构域与小核糖核酸病毒前导蛋白酶的相似性。冠状病毒科特有的ADRP（APPR-1″）结构域也位于BoTV pp 1a中。此外，还鉴定了几个典型的巢病毒复制酶的疏水结构域。在pp 1b序列的聚合酶和解旋酶结构域进行了鉴定，以及序列预测参与核糖体移码，包括保守的滑序列UUUAAAC和两个潜在的假结结构。
Viruses in the family Coronaviridae have elicited new interest, with the outbreaks caused by SARS-HCoV in 2003 and the recent discovery of a new human coronavirus, HCoV-NL63. The genus Torovirus, within the family Coronaviridae, is less well characterized, in part because toroviruses cannot yet be grown in cell culture (except for the Berne virus). In this study, we determined the sequence of the complete genome of Breda-1 (BoTV-1), a bovine torovirus. This is the first complete torovirus genome sequence to be reported. BoTV-1 RNA was amplified using long RT-PCR and the amplicons sequenced. The genome has a length of 28.475 kb and consisted mainly of the replicase gene (∼20.2 kb) which contains two large overlapping ORFs, ORF1a and ORF1b, encoding polyproteins pp1a and pp1b, respectively. Sequence analysis identified conserved domains within the predicted sequences of pp1a and pp1b. Sequence alignments and protein secondary structure prediction data suggest the presence of a 3C-like serine protease domain with similarity to the arterivirus 3C-like serine protease and a single papain-like cysteine protease domain with similarity to the picornavirus leader protease. The ADRP (APPR-1″) domain – unique to the Coronaviridae – was also located in BoTV pp1a. In addition, several hydrophobic domains were identified that are typical of a nidovirus replicase. Within the pp1b sequence the polymerase and helicase domains were identified, as well as sequences predicted to be involved in ribosomal frameshifting, including the conserved slippery sequence UUUAAAC and two potential pseudoknot structures.
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DOI：
10.1074/jbc.274.21.14918
发表时间：
1999-05-21
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Herold J;Siddell SG;Gorbalenya AE
通讯作者：
Gorbalenya AE
影响因子：
2.7
作者：
González JM;Gomez-Puertas P;Cavanagh D;Gorbalenya AE;Enjuanes L
通讯作者：
Enjuanes L
影响因子：
5.4
作者：
Cornelissen, LAHM;Wierda, CMH;deGroot, RJ
通讯作者：
deGroot, RJ
影响因子：
1
作者：
Hoet, AE;Smiley, J;Saif, LJ
通讯作者：
Saif, LJ
影响因子：
1.5
作者：
Hoet, AE;Nielsen, PR;Saif, LJ
通讯作者：
Saif, LJ